Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13430
Title: Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
Authors: Guedes, Carlos Eduardo Sampaio
Lima, José Geraldo Bomfim
Helfer, Emmanuèle
Veras, Patrícia Sampaio Tavares
Viallat, Annie
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Aix-Marseille Université. CNRS. Marseille, France
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Aix-Marseille Université. CNRS. Marseille, France
Abstract: After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate. Here, using a variant of the ‘inverted emulsion’ method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs. We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome. L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C. At 37°C and pH 5.5, parasites survived 48h. This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell. This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
keywords: Leishmania
Macrófagos
Leishmania amazonensis
Mamíferos
Fagocitose
Issue Date: 2015
Publisher: Public Library of Science
Citation: GUEDES, C. E. S. et al. Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. Plos One, v. 10, n. 8, p. e0134925, 2015.
DOI: 10.1371/journal.pone.0134925
ISSN: 1932-6203
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Guedes, Carlos ES. Encapsulation of living....pdf1.96 MBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.