Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13520
Title: Aspidosperma (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part II: experimental studies withAspidosperma ramiflorum in vivo and in vitro.
Authors: Aguiar, Anna Caroline Campos
Cunha, Ananda de Castro
Cerávolo, Isabela Penna
Gonçalves, Regina Aparecida Correia
Oliveira, Arildo José Braz de
Krettli, Antoniana Ursine
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil
Universidade Estadual de Maringá. Departamento de Farmácia. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Universidade Estadual de Maringá. Departamento de Farmácia. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil
Universidade Estadual de Maringá. Departamento de Farmácia. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil
Abstract: Several species of Aspidosperma plants are used to treat diseases in the tropics, including Aspidosperma ramiflorum, which acts against leishmaniasis, an activity that is experimentally confirmed. The species, known as guatambu yellow, yellow peroba, coffee peroba matiambu, grows in the Atlantic Forest of Brazil in the South to the Southeast regions. Through a guided biofractionation of A. ramiflorum extracts, the plant activity against Plasmodium falciparum was evaluated in vitro for toxicity towards human hepatoma G2 cells, normal monkey kidney cells and nonim mortalised human monocytes isolated from peripheral blood. Six of the seven extracts tested were active at low doses (half-maximal drug inhibitory concentration < 3.8 μg/mL); the aqueous extract was inactive. Overall, the plant extracts and the purified compounds displayed low toxicity in vitro. A non soluble extract fraction and one purified alkaloid isositsirikine (compound 5) displayed high selectivity indexes (SI) (= 56 and 113, respectively), where as compounds 2 and 3 were toxic (SI < 10). The structure, activity and low toxicity of isositsirikine in vitro are described here for the first time in A. ramiflorum, but only the neutral and precipitate plant fractions were tested for activity, which caused up to 53% parasitaemia inhibition of Plasmodium berghei in mice with blood-induced malaria. This plant species is likely to be useful in the further development of an antimalarial drug, but its pharmacological evaluation is still required.
Keywords: Aspidosperma ramiflorum
ethnopharmacology - antimalarials
P. falciparum
medicinal plants
Issue Date: 2015
Publisher: Fundação Oswaldo Cruz
Citation: AGUIAR, Anna Caroline Campos et al. Aspidosperma (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part II: experimental studies withAspidosperma ramiflorum in vivo and in vitro. Mem. Inst. Oswaldo Cruz, v. 110, n. 7, p. 906-913, 2015.
DOI: 10.1590/0074-02760150188
ISSN: 1678-8060
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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