Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13569
Title: Previous ingestion of lactococcus lactis by ethanol-treated mice preserves antigen presentation hierarchy in the gut and oral tolerance susceptibility
Authors: Carvalho, Débora Moreira Alvarenga de
Perez, Denise Alves
Santos, Ana Cristina Gomes
Miyoshi, Anderson
Azevedo, Vasco
Reis, Jordana Grazziela Alves Coelho dos
Martins Filho, Olindo Assis
Faria, Ana Maria Caetano de
Machado, Denise Carmona Cara
Andrade, Marileia Chaves
Affilliation: Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brasil
Universidade Estadual de Montes Claros. Hospital Universitário Clemente de Faria. Montes Claros, MG, Brazil
Abstract: BACKGROUND: Ethanol (EtOH) consumption is able to disturb the ovalbumin (OVA)-oral tolerance induction by interfering on the function of antigen presenting cells (APC), down-regulating dendritic cells (DCs) and macrophages and up-regulating B-lymphocytes and their function, which results in an overall allergic-type immune status. In this study, the potential of a priori administration of Lactococcus lactis (LL) in avoiding loss of oral tolerance in EtOH-treated mice was investigated. METHODS: Female C57BL/6 mice received, by oral route, ad libitum wild-type (WT) LL or heat-shock protein producer (Hsp65) LL for 4 consecutive days. Seven days later, mice were submitted to short-term high-dose EtOH treatment. After 24 hours, stomach, intestine, spleen, mesenteric lymph nodes (mLN) specimens were collected for biomarkers analysis. Following EtOH-treatment protocol, a group of animals underwent single-gavage OVA-tolerance protocol and sera samples collected for antibody analysis. RESULTS: The ingestion of WT LL or Hsp65 LL is able to restore oral tolerance to OVA in EtOH-treated mice, by reducing local and systemic allergic outcomes such as gastric mast cells and gut-interleukin-4, as well as serum IgE. WT LL treatment prevents the decrease of mLN regulatory T cells induced by the EtOH treatment. Moreover, LL treatment preserves APC hierarchy and antigen presentation commitment in EtOH-treated mice, with conserved DC and macrophage activity over B lymphocytes in mLN and preserved macrophage activity over DC and B-cell subsets in the spleen. CONCLUSIONS: The present findings suggest that a priori ingestion of LL preserves essential mechanisms associated with oral tolerance induction that are disturbed by EtOH ingestion. Maintenance of mucosal homeostasis by preserving APC hierarchy and antigen presentation commitment could be associated with T-regulatory subset activities in the gastrointestinal
Keywords: Antigen Presentation
Ethanol
Intestine
Lactococcus lactis
Oral Tolerance
Issue Date: 2015
Publisher: Willey Online Library
Citation: CARVALHO, Débora Moreira Alvarenga de et al. Previous Ingestion of Lactococcus lactis by Ethanol-Treated Mice Preserves Antigen Presentation Hierarchy in the Gut and Oral Tolerance Susceptibility. Alcohol Clin Exp Res., vol. 39, n. 8, p. 1453-64.
DOI: 10.1111/acer.12770
ISSN: 1530-0277
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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