Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14033
Title: A Novel Phospholipase A2 (D49) from the Venom of the Crotalus oreganus abyssus (North American Grand Canyon Rattlesnake)
Authors: Martins, W.
Baldasso, P. A.
Honório, K. M.
Maltarollo, V. G.
Ribeiro, R. I. M. A.
Carvalho, B. M. A.
Soares, Andreimar Martins.
Calderon, Leonardo de Azevedo.
Stábeli, Rodrigo Guerino.
Caballol, M. A. O.
Acosta, G.
Oliveira, E.
Marangoni, S.
Albericio, F.
Silva, S. L. Da
Affilliation: Federal University of São João Del Rei. Campus Alto Paraopeba. Department of Chemistry, Biochemistry and Bioprocess Engineering. Ouro Branco, MG, Brazil.
State University of Campinas. Department of Biochemistry of Biology Institute. Campinas, SP, Brazil.
University of São Paulo. School of Arts, Sciences and Humanities. São Paulo, SP, Brazil. / Federal University of ABC. Center of Natural Sciences and Humanities. Santo André, SP, Brazil.
Federal University of ABC. Center of Natural Sciences and Humanities. Santo André, SP, Brazil.
Federal University of São João Del Rei. Campus Alto Paraopeba. Department of Chemistry, Biochemistry and Bioprocess Engineering. Ouro Branco, MG, Brazil.
Federal University of São João Del Rei. Campus Alto Paraopeba. Department of Chemistry, Biochemistry and Bioprocess Engineering. Ouro Branco, MG, Brazil.
Oswaldo Cruz Foundation. Center of Biomolecules Study Applied to Health. Federal University of Rondônia. Medicine Department. Porto Velho, RO, Brazil.
Oswaldo Cruz Foundation. Center of Biomolecules Study Applied to Health. Federal University of Rondônia. Medicine Department. Porto Velho, RO, Brazil.
Oswaldo Cruz Foundation. Center of Biomolecules Study Applied to Health. Federal University of Rondônia. Medicine Department. Porto Velho, RO, Brazil.
University of Barcelona. Proteomics Platform. Barcelona Science Park. Barcelona, Spain.
University of Barcelona. Institute for Research in Biomedicine, Barcelona Science Park. Barcelona, Spain. / University of Barcelona. Barcelona Science Park. Barcelona, Spain.
University of Barcelona. Proteomics Platform. Barcelona Science Park. Barcelona, Spain.
State University of Campinas. Department of Biochemistry of Biology Institute. Campinas, SP, Brazil.
University of Barcelona. Institute for Research in Biomedicine, Barcelona Science Park. Barcelona, Spain. / University of Barcelona. Barcelona Science Park. Barcelona, Spain. / University of Barcelona. Department of Organic Chemistry. Barcelona, Spain. / University of KwaZulu-Natal. School of Chemistry and Physics. Durban, South Africa.
Federal University of São João Del Rei. Campus Alto Paraopeba. Department of Chemistry, Biochemistry and Bioprocess Engineering. Ouro Branco, MG, Brazil.
Abstract: Currently, Crotalus viridis was divided into two species: Crotalus viridis and Crotalus oreganus. The current classification divides “the old” Crotalus viridis into two new and independent species: Crotalus viridis (subspecies: viridis and nuntius) and Crotalus oreganus (subspecies: abyssus, lutosus, concolor, oreganus, helleri, cerberus, and caliginis). The analysis of a product from cDNA (E6d), derived from the gland of a specie Crotalus viridis viridis, was found to produce an acid phospholipase A2. In this study we isolated and characterized a PLA2 (D49) fromCrotalus oreganus abyssus venom. Our studies show that the PLA2 produced from the cDNA of Crotalus viridis viridis (named E6d) is exactly the same PLA2 primary sequence of amino acids isolated from the venom of Crotalus oreganus abyssus. Thus, the PLA2 from E6d cDNA is actually the same PLA2 presented in the venom of Crotalus oreganus abyssus and does not correspond to the venom from Crotalus viridis viridis. These facts highlight the importance of performing more studies on subspecies of Crotalus oreganus and Crotalus viridis, since the old classification may have led to mixed results or mistaken data.
Keywords: Phospholipase A2 (D49)
Crotalus oreganus abyssus
Grand Canyon
Venom
Issue Date: 2014
Publisher: BioMed Research International
Citation: SOARES, A. M., et al. A novel phospholipase A2 (D49) from the venom of the Crotalus oreganus abyssus (North American Grand Canyon Rattlesnake). Journal of Biomedicine and Biotechnology (Print), v. 2014, p. 1-11, 2014.
DOI: http://dx.doi.org/10.1155/2014/654170
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos

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