Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14081
Title: Proteome profiling of human cutaneous leishmaniasis lesion.
Authors: Santos, Claire da Silva
Attarha, Sanaz
Saini, Ravi Kanth
Boaventura, Viviane
Costa, Jackson Mauricio Lopes
Khouri, Ricardo
Barral Netto, Manoel
Brodskyn, Claudia Ida
Souchelnytskyi, Serhiy
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil
Karolinska Institutet. Department of Oncology-Pathology. Stockholm, Sweden
Karolinska Institutet. Department of Oncology-Pathology. Stockholm, Sweden
Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Karolinska Institutet. Department of Oncology-Pathology. Stockholm, Sweden
Abstract: In this study, we used proteomics and biological network analysis to evaluate the potential biological processes and components present in the identified proteins of biopsies from cutaneous leishmaniasis (CL) patients infected by Leishmania braziliensis in comparison with normal skin. We identified 59 proteins differently expressed in samples from infected and normal skin. Biological network analysis employing identified proteins showed the presence of networks that may be involved in the cell death mediated by cytotoxic T lymphocytes. After immunohistochemical analyses, the expression of caspase-9, caspase-3, and granzyme B was validated in the tissue and positively correlated with the lesion size in CL patients. In conclusion, this work identified differentially expressed proteins in the inflammatory site of CL, revealed enhanced expression of caspase-9, and highlighted mechanisms associated with the progression of tissue damage observed in lesions.
DeCS: Leishmaniose Cutânea/metabolismo
Proteoma
Caspase 3/análise
Caspase 9/análise
Granzimas/análise
Humanos
Imuno-Histoquímica
Leishmaniose Cutânea/patologia
Pele/patologia
Issue Date: 2015
Publisher: Elsevier
Citation: SANTOS, C. S. et al. Proteome profiling of human cutaneous leishmaniasis lesion. Journal of Investigative Dermatology, v. 135, n. 2, p. 400-410, 2015.
DOI: 10.1038/jid.2014.396
ISSN: 1523-1747
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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