Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14265
Title: Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy
Authors: Pinto, Lorena Ana
Meira, Cássio Santana
Villarreal, Cristiane Flora
Santos, Marcos André Vannier dos
Souza, Claudia V.C. de
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Castro Filho, Bernardo Galvão
Soares, Milena Botelho Pereira
Grassi, Maria Fernanda Rios
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center of Biotechnology and Cell Therapy. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil
Abstract: Human T-lymphotropic virus type 1 (HTLV-1) induces a strong activation of the immune system, especially in individuals with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. The present study aimed to investigate the effects of physalin F on peripheral blood mononuclear cells (PBMC) of HAM/TSP subjects. A concentration-dependent inhibition of spontaneous proliferation of PBMC from HAM/TSP subjects was observed in the presence of physalin F, as evaluated by 3H-thymidine uptake. The IC50 for physalin F was 0.97 0.11 mM. Flow cytometry analysis using Cytometric Bead Array (CBA) showed that physalin F (10 mM) significantly reduced the levels of IL-2, IL-6, IL-10, TNF-a and IFN-g, but not IL-17A, in supernatants of PBMC cultures. Next, apoptosis induction was addressed by using flow cytometry to evaluate annexin V expression. Treatment with physalin F (10 mM) increased the apoptotic population of PBMC in HAM/TSP subjects. Transmission electron microscopy analysis of PBMC showed that physalin F induced ultrastructural changes, such as pyknotic nuclei, damaged mitochondria, enhanced autophagic vacuole formation, and the presence of myelin-like figures. In conclusion, physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by HTLV-1 infection
Keywords: Physalin F
HTLV-1
PBMC
Immunosuppressive activity
Apoptosis
Issue Date: 2016
Publisher: Elsevier
Citation: PINTO, L. A. et al. Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy. Biomedicine and Pharmacotherapy, v. 79, p. 129-134, 2016.
DOI: dx.doi.org/10.1016/j.biopha.2016.01.041
ISSN: 0753-3322
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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