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THE EFFECTS OF DASATINIB IN EXPERIMENTAL ACUTE RESPIRATORY DISTRESS SYNDROME DEPEND ON DOSE AND ETIOLOGY
Cytokines
Histology
Lung mechanics
Acute respiratory distress syndrome
Toll like receptor-4
Author
Affilliation
Abstract
Background/Aims: Evidence suggests that tyrosine-kinase inhibitors may attenuate lung
inflammation and fibrosis in experimental acute respiratory distress syndrome (ARDS). We
hypothesized that dasatinib, a tyrosine-kinase inhibitor, might act differently depending on
the ARDS etiology and the dose. Methods: C57/BL6 mice were divided to be pre-treated
with dasatinib (1mg/kg or 10mg/kg) or vehicle (1% dimethyl-sulfoxide) by oral gavage.
Thirty-minutes after pre-treatment, mice were subdivided into control (C) or ARDS groups.
ARDS animals received Escherichia coli lipopolysaccharide intratracheally (ARDSp) or
intraperitoneally (ARDSexp). A new dose of dasatinib or vehicle was administered at 6 and 24h.
Results: Forty-eight hours after ARDS induction, dasatinib 1mg/kg yielded: improved lung
morphofunction and reduced cells expressing toll-like receptor (TLR)-4 in lung, independent
of ARDS etiology; reduced neutrophil and levels of interleukin (IL)-6, IL-10 and transforming
growth factor (TGF)-β in ARDSp. The higher dose of dasatinib caused no changes in lung
mechanics, diffuse alveolar damage, neutrophil, or cells expressing TLR4, but increased IL-6,
vascular endothelial growth factor (VEGF), and cells expressing Fas receptor in lung in ARDSp.
In ARDSexp, it improved lung morphofunction, increased VEGF, and reduced cells expressing
TLR4. Conclusion: Dasatinib may have therapeutic potential in ARDS independent of etiology,
but careful dose monitoring is required.
Keywords in Portuguese
Síndrome da insuficiência respiratória agudaKeywords
DasatinibCytokines
Histology
Lung mechanics
Acute respiratory distress syndrome
Toll like receptor-4
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