Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14321
Title: The Flagellar Protein FLAG1/SMP1 is a Candidate for Leishmania–Sand Fly Interaction
Authors: Di-Biasi, Tatiana
Lobo, Amanda R.
Nascimento, Luanda M.
Córdova-Rojas, Jose L.
Pestana, Karen
Marín-Villa, Marcel
Tempone, Antonio J.
Telleria, Erich L.
Ramalho-Ortigão, Marcelo
McMahon-Pratt, Diane
Traub-Cseko, Yara M.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Yale University School of Public Health. New Haven, Connecticut.USA.
Yale University School of Public Health. New Haven, Connecticut.USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Kansas State University. Department of Entomology. Manhattan, Kansas, USA.
Yale University School of Public Health. New Haven, Connecticut.USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
Abstract: Leishmaniasis is a serious problem that affects mostly poor countries. Various species of Leishmania are the agents of the disease, which take different clinical manifestations. The parasite is transmitted by sandflies, predominantly from the Phlebotomus genus in the Old World and Lutzomyia in the New World. During development in the gut, Leishmania must survive various challenges, which include avoiding being expelled with blood remnants after digestion. It is believed that attachment to the gut epithelium is a necessary step for vector infection, and molecules from parasites and sand flies have been implicated in this attachment. In previous work, monoclonal antibodies were produced against Leishmania. Among these an antibody was obtained against Leishmania braziliensis flagella, which blocked the attachment of Leishmania panamensis flagella to Phlebotomus papatasi guts. The protein recognized by this antibody was identified and named FLAG1, and the complete FLAG1 gene sequence was obtained. This protein was later independently identified as a small, myristoylated protein and called SMP1, so from now on it will be denominated FLAG1/SMP1. The FLAG1/SMP1 gene is expressed in all developmental stages of the parasite, but has higher expression in promastigotes. The anti-FLAG1/SMP1 antibody recognized the flagellum of all Leishmania species tested and generated the expected band by western blots. This antibody was used in attachment and infection blocking experiments. Using the New World vector Lutzomyia longipalpis and Leishmania infantum chagasi, no inhibition of attachment ex vivo or infection in vivo was seen. On the other hand, when the Old World vectors P. papatasi and Leishmania major were used, a significant decrease of both attachment and infection were seen in the presence of the antibody. We propose that FLAG1/SMP1 is involved in the attachment/infection of Leishmania in the strict vector P. papatasi and not the permissive vector L. longipalpis.
Keywords: Leishmania
Lutzomyia longipalpis
Phlebotomus papatasi
Flagellum
FLAG1/SMP1
Leishmaniasis
Sand fly
Vector–parasite interaction
keywords: Interação vetor-parasita
Flagelo
DeCS: Leishmania
Phlebotomus
Leishmaniose
Flebotomíneos
Issue Date: 2015
Publisher: Mary Ann Liebert
Citation: DI-BIASI, Tatiana; et al. The Flagellar Protein FLAG1/SMP1 is a Candidate for Leishmania–Sand Fly Interaction. Vector-Borne and Zoonotic Diseases, v.15, n.3, p.202-209, Mar. 2015.
DOI: 10.1089/vbz.2014.1736
ISSN: 1530-3667
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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