Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14515
Title: Molecular Individual-Based Approach on Triatoma brasiliensis: Inferences on Triatomine Foci, Trypanosoma cruzi Natural Infection Prevalence, Parasite Diversity and Feeding Sources
Authors: Almeida, Carlos Eudardo
Faucher, Leslie
Lavina, Morgane
Costa, Jane
Harry, Myriam
Affilliation: Universidade Estadual Paulista (UNESP). Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas. Araraquara, SP, Brasil / UMR EGCE (Evolution, Genome, Comportment, Ecologie), CNRS-IRD-Univ. Paris-Sud, IDEEV, Université Paris-Saclay, Gif-sur-Yvette Cedex, France / Universidade Federal da Paraíba. Programa de Pós-Graduação em Ecologia e Monitoramento Ambiental – PPGEMA. PB, Brasil .
UMR EGCE (Evolution, Genome, Comportment, Ecologie), CNRS-IRD-Univ. Paris-Sud, IDEEV. Université Paris-Saclay. Gif-sur-Yvette Cedex, France.
Laboratório de Biodiversidade Entomológica
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil
UMR EGCE (Evolution, Genome, Comportment, Ecologie), CNRS-IRD-Univ. Paris-Sud, IDEEV. Université Paris-Saclay. Gif-sur-Yvette Cedex, France / Université Paris-Sud, Orsay, France.
Abstract: We used an individual-based molecular multisource approach to assess the epidemiological importance of Triatoma brasiliensis collected in distinct sites and ecotopes in Rio Grande do Norte State, Brazil. In the semi-arid zones of Brazil, this blood sucking bug is the most important vector of Trypanosoma cruzi—the parasite that causes Chagas disease. First, cytochrome b (cytb) and microsatellite markers were used for inferences on the genetic structure of five populations (108 bugs). Second, we determined the natural T. cruzi infection prevalence and parasite diversity in 126 bugs by amplifying a mini-exon gene from triatomine gut contents. Third, we identified the natural feeding sources of 60 T. brasiliensis by using the blood meal content via vertebrate cytb analysis. Demographic inferences based on cytb variation indicated expansion events in some sylvatic and domiciliary populations. Microsatellite results indicated gene flow between sylvatic and anthropic (domiciliary and peridomiciliary) populations, which threatens vector control efforts because sylvatic population are uncontrollable. A high natural T. cruzi infection prevalence (52–71%) and two parasite lineages were found for the sylvatic foci, in which 68% of bugs had fed on Kerodon rupestris (Rodentia: Caviidae), highlighting it as a potential reservoir. For peridomiciliary bugs, Galea spixii (Rodentia: Caviidae) was the main mammal feeding source, which may reinforce previous concerns about the potential of this animal to link the sylvatic and domiciliary T. cruzi cycles.
Keywords: Triatoma brasiliensis
Rio Grande do Norte
Trypanosoma cruzi
Chagas Disease
Vector control
Molecular Individual-Based Approach
keywords: Doença de Chagas
Tripanosoma cruzi
Abordagem Molecular baseado no indivíduo
Controle de vetores
Rio Grande do Norte
Issue Date: 2016
Publisher: Public Library of Science
Citation: ALMEIDA, Carlos Eduardo; et al. Molecular Individual-Based Approach on Triatoma brasiliensis: Inferences on Triatomine Foci, Trypanosoma cruzi Natural Infection Prevalence, Parasite Diversity and Feeding Sources. PLoS Negl Trop Dis., v.10, n.2, e0004447, 19p, Feb. 2016.
DOI: 10.1371/ journal.pntd.0004447
ISSN: 1935-2727
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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