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LOW IMPACT TO FIXED CELL PROCESSING AIMING TRANSMISSION ELECTRON MICROSCOPY
Barth, Ortrud Monika et al. | Date Issued: 2016
Author
Barth, Ortrud Monika
Silva, Marcos Alexandre Nunes da
Vieira, Debora Ferreira Barreto
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
Abstract
In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.
Keywords in Portuguese
Baixo impacto
Fixação das células
 
Keywords
Cell fixation
Transmission electron microscopy
Low impact
DENV
 
DeCS
Microscopia Eletrônica de Transmissão
Publisher
Fundação Oswaldo Cruz
Citation
BARTH, Ortud Monika; SILVA, Marcos Alexandre Nunes da; VIEIRA, Debora Fereira Barreto. Low impact to fixed cell processing aiming transmission electron microscopy. Mem. Inst. Oswaldo Cruz, v.111, n.6, p.411-413, June 2016.
DOI
10.1590/0074-02760150433
ISSN
0074-0276