Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14642
Title: Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
Authors: Calegar, Deiviane Aparecida
Nunes, Beatriz Coronato
Monteiro, Kerla Joeline Lima
Santos, Jéssica Pereira dos
Toma, Helena Keiko
Gomes, Tais Ferreira
Lima, Marli Maria
Bóia, Márcio Neves
Carvalho-Costa, Filipe Anibal
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.
Universidade Federal do Rio de Janeiro. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.
Abstract: This study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods. E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishable Entamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% to E. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskii complex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission.
Keywords: Parasites
Protozoa
entamoebiasis
Epidemiology
Brazil
keywords: Parasitos
Parasitologia
Entamebíase
Epidemiologia
Brasil
Issue Date: 2016
Publisher: Fundação Oswaldo Cruz
Citation: CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.
DOI: 10.1590/0074-02760150383
ISSN: 0074-0276
Copyright: open access
Appears in Collections:PI - Artigos de Periódicos
IOC - Artigos de Periódicos

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