| Author | Avila, Herbert A. | |
| Author | Pereira, Jose Borges | |
| Author | Thiemann, Otavio | |
| Author | Paiva, Eugenia de | |
| Author | Degrave, Wim | |
| Author | Morel, Carlos Medicis | |
| Author | Simpson, Larry | |
| Access date | 2016-06-29T14:58:50Z | pt_BR |
| Access date | 2016-07-07T12:05:14Z | |
| Available date | 2016-06-29T14:58:50Z | pt_BR |
| Available date | 2016-07-07T12:05:14Z | |
| Document date | 1993 | |
| Citation | AVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993. | pt_BR |
| ISSN | 0095-1137 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14660 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Title | Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis | pt_BR |
| Type | Article | |
| Abstract | A panel of 114 blood samples from chronic chagasic patients and nonchagasic patients was screened for
Trypanosoma cruzi by xenodiagnostic, serologic, and polymerase chain reaction (PCR) amplification tests.
Blood samples were preserved in a guanidine-EDTA buffer, and total blood DNA was isolated after chemical
nuclease cleavage with 1,10-phenanthroline-copper ion and used as a template for PCR amplification of the
conserved and variable regions of T. cruzi minicircle molecules. The PCR products were screened by Southern
blot hybridization with a digoxigenin-labeled oligonucleotide probe specific for the conserved region of the
minicircle. The method showed a sensitivity of 100%o compared with the serologic test. In addition, all of the
serology-positive, xenodiagnosis-negative samples were positive by PCR. This demonstrates that PCR
amplification of T. cruzi kinetoplast minicircle DNA could replace xenodiagnosis for evaluation of parasitemia
in chronic chagasic patients and could serve as a complement for serologic testing in the screening of blood bank
donors. | pt_BR |
| Affilliation | University of California. Department of Biology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of California. Department of Biology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | University of California. Department of Biology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of California. Department of Biology. Los Angeles, CA, USA / University of California. Molecular Biology Institute. Los Angeles, CA, USA / University of California. Howard Hughes Medical Institute. Los Angeles, CA, USA. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Chronic Chagasic Patients | pt_BR |
| Subject | Polymerase Chain Reaction | pt_BR |
| Subject | Kinetoplast Minicircle DNA | pt_BR |
| Subject | Serology | pt_BR |
| Subject | Xenodiagnosis | pt_BR |
| DeCS | Trypanosoma cruzi | pt_BR |
| DeCS | Xenodiagnóstico | pt_BR |
| DeCS | Doença de Chagas | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Sorologia | pt_BR |
| DeCS | DNA de Cinetoplasto | pt_BR |
| e-ISSN | 1098-660X | pt_BR |
