Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14660
Title: Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
Authors: Avila, Herbert A.
Pereira, Jose Borges
Thiemann, Otavio
Paiva, Eugenia de
Degrave, Wim
Morel, Carlos Medicis
Simpson, Larry
Affilliation: University of California. Department of Biology. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical Rio de Janeiro, RJ, Brasil.
University of California. Department of Biology. Los Angeles, CA, USA.
University of California. Department of Biology. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
University of California. Department of Biology. Los Angeles, CA, USA / University of California. Molecular Biology Institute. Los Angeles, CA, USA / University of California. Howard Hughes Medical Institute. Los Angeles, CA, USA.
Abstract: A panel of 114 blood samples from chronic chagasic patients and nonchagasic patients was screened for Trypanosoma cruzi by xenodiagnostic, serologic, and polymerase chain reaction (PCR) amplification tests. Blood samples were preserved in a guanidine-EDTA buffer, and total blood DNA was isolated after chemical nuclease cleavage with 1,10-phenanthroline-copper ion and used as a template for PCR amplification of the conserved and variable regions of T. cruzi minicircle molecules. The PCR products were screened by Southern blot hybridization with a digoxigenin-labeled oligonucleotide probe specific for the conserved region of the minicircle. The method showed a sensitivity of 100%o compared with the serologic test. In addition, all of the serology-positive, xenodiagnosis-negative samples were positive by PCR. This demonstrates that PCR amplification of T. cruzi kinetoplast minicircle DNA could replace xenodiagnosis for evaluation of parasitemia in chronic chagasic patients and could serve as a complement for serologic testing in the screening of blood bank donors.
Keywords: Trypanosoma cruzi
Chronic Chagasic Patients
Polymerase Chain Reaction
Kinetoplast Minicircle DNA
Serology
Xenodiagnosis
DeCS: Trypanosoma cruzi
Xenodiagnóstico
Doença de Chagas
Reação em Cadeia da Polimerase
Sorologia
DNA de Cinetoplasto
Issue Date: 1993
Publisher: American Society for Microbiology
Citation: AVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993.
DOI: PMCID: PMC265772
ISSN: 0095-1137
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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