| Author | Wincker, Patrick | |
| Author | Bosseno, Marie-France | |
| Author | Britto, Constança | |
| Author | Yaksic, Nina | |
| Author | Cardoso, Maria Angélica | |
| Author | Morel, Carlos Medicis | |
| Author | Brenière, Simone Frédérique | |
| Access date | 2016-06-29T15:43:03Z | pt_BR |
| Access date | 2016-07-07T12:13:56Z | |
| Available date | 2016-06-29T15:43:03Z | pt_BR |
| Available date | 2016-07-07T12:13:56Z | |
| Document date | 1994 | |
| Citation | WINCKER, Patrick et al. High correlation between chagas'disease serology and pcr-based detection of trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiology Letters, v. 124, p. 419-424, 1994. | pt_BR |
| ISSN | 0378-1097 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14675 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | |
| Title | High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1574-6968.1994.tb07318.x | pt_BR |
| Abstract | The detection of Tr)tpunosomu crirzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially
powerful tool for the parasitological diagnosis of Chagas’ disease. We have applied this technique in a field situation in Bolivia,
where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28
serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR,
including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a
very useful complement to serology in Chagas’ disease diagnosis in Bolivia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Mayor de San Andres. Instituto Boliviano de Biologia de Altura. La Paz, Bolivia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Chagas`Disease | pt_BR |
| Subject | Kinetoplast DNA | pt_BR |
| Subject | PCR Diagonosis | pt_BR |
| Subject | Bolivia | pt_BR |
| DeCS | Trypanosoma cruzi | pt_BR |
| DeCS | Doença de Chagas | pt_BR |
| DeCS | DNA de Cinetoplasto | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Diagnóstico | pt_BR |
| DeCS | Bolívia | pt_BR |
| e-ISSN | 1574-6968 | pt_BR |
