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HIGH CORRELATION BETWEEN CHAGAS’ DISEASE SEROLOGY AND PCR-BASED DETECTION OF TRYPANOSOMA CRUZI KINETOPLAST DNA IN BOLIVIAN CHILDREN LIVING IN AN ENDEMIC AREA
Doença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
Diagnóstico
Bolívia
Author
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Abstract
The detection of Tr)tpunosomu crirzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially
powerful tool for the parasitological diagnosis of Chagas’ disease. We have applied this technique in a field situation in Bolivia,
where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28
serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR,
including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a
very useful complement to serology in Chagas’ disease diagnosis in Bolivia.
DeCS
Trypanosoma cruziDoença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
Diagnóstico
Bolívia
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