Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14675
Title: High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area
Authors: Wincker, Patrick
Bosseno, Marie-France
Britto, Constança
Yaksic, Nina
Cardoso, Maria Angélica
Morel, Carlos Medicis
Brenière, Simone Frédérique
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Universidad Mayor de San Andres. Instituto Boliviano de Biologia de Altura. La Paz, Bolivia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.
Abstract: The detection of Tr)tpunosomu crirzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas’ disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas’ disease diagnosis in Bolivia.
Keywords: Trypanosoma cruzi
Chagas`Disease
Kinetoplast DNA
PCR Diagonosis
Bolivia
DeCS: Trypanosoma cruzi
Doença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
Diagnóstico
Bolívia
Issue Date: 1994
Publisher: Wiley
Citation: WINCKER, Patrick et al. High correlation between chagas'disease serology and pcr-based detection of trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiology Letters, v. 124, p. 419-424, 1994.
DOI: 10.1111/j.1574-6968.1994.tb07318.x
ISSN: 0378-1097
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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