| Author | Contreras, Victor T. | |
| Author | Salles, Jussara M. | |
| Author | Thomas, Neide | |
| Author | Morel, Carlos Medicis | |
| Author | Goldenberg, Samuel | |
| Access date | 2016-06-28T15:57:55Z | pt_BR |
| Access date | 2016-07-07T12:15:51Z | |
| Available date | 2016-06-28T15:57:55Z | pt_BR |
| Available date | 2016-07-07T12:15:51Z | |
| Document date | 1985 | |
| Citation | CONTRERAS, Victor T. et al. In vitro differentatiation of Trypanosoma Cruzi under chemically defined conditions. Molecular and Biochemical Parasitology, v.16, p.315-327 , 1985. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14681 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Title | In vitro differentiation of Trypanosoma cruzi under chemically defined conditions | pt_BR |
| Type | Article | |
| Abstract | Metacyclic trypomastigotes of Trypanosoma cruzi have been obtained in chemically defined axenic
culture. The differentiating medium, composed of artificial triatomine urine supplemented with proline,
allows high yields of metacyclic trypomastigotes after 72-h incubation of T. cruzi cells at 27°C. Morphological
differentiation of the parasites is gradual under these chemically defined conditions and is preceded by
the expression of stage-specific polypeptides. The yield of in vitro-induced metacyclic trypomastigotes
depends upon the age of the epimastigote culture, the size of the inoculum and the depth of the medium.
Metacyclic trypomastigotes differentiated in vitro from the Dm 28c clone of T. cruzi are both resistant to
complement lysis and to macrophage digestion. They are able to infect mice with an efficiency similar to that
obtained for natural metacyclic trypomastigotes obtained from triatomine excreta. | pt_BR |
| Affilliation | Universidad de Carabobo. Valencia, Venezuela. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Niteroi, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Metacyclogenesis | pt_BR |
| Subject | In vitro differentiation | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| e-ISSN | 1872-9428 | pt_BR |
