Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14681
Title: In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
Authors: Contreras, Victor T.
Salles, Jussara M.
Thomas, Neide
Morel, Carlos Medicis
Goldenberg, Samuel
Affilliation: Universidad de Carabobo. Valencia, Venezuela.
Universidade Federal Fluminense. Niteroi, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Metacyclic trypomastigotes of Trypanosoma cruzi have been obtained in chemically defined axenic culture. The differentiating medium, composed of artificial triatomine urine supplemented with proline, allows high yields of metacyclic trypomastigotes after 72-h incubation of T. cruzi cells at 27°C. Morphological differentiation of the parasites is gradual under these chemically defined conditions and is preceded by the expression of stage-specific polypeptides. The yield of in vitro-induced metacyclic trypomastigotes depends upon the age of the epimastigote culture, the size of the inoculum and the depth of the medium. Metacyclic trypomastigotes differentiated in vitro from the Dm 28c clone of T. cruzi are both resistant to complement lysis and to macrophage digestion. They are able to infect mice with an efficiency similar to that obtained for natural metacyclic trypomastigotes obtained from triatomine excreta.
Keywords: Metacyclogenesis
In vitro differentiation
Trypanosoma cruzi
keywords: Trypanosoma cruzi
Issue Date: 1985
Publisher: Elsevier
Citation: CONTRERAS, Victor T. et al. In vitro differentatiation of Trypanosoma Cruzi under chemically defined conditions. Molecular and Biochemical Parasitology, v.16, p.315-327 , 1985.
ISSN: 0166-6851
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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