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CHARACTERIZATION OF TWO ISOLATES OF TRYPANOSOMA CRUZI OBTAINED FROM THE PATIENT BERENICE, THE FIRST HUMAN CASE OF CHAGAS’ DISEASE DESCRIBED BY CARLOS CHAGAS IN 1909
Lana, Marta de et al. | Date Issued: 1996
Author
Lana, Marta de
Chiari, Clea A.
Chiari, Egler
Morel, Carlos Medicis
Gonçalves, Antonio M.
Romanha, Álvaro J.
Affilliation
Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.
Abstract
Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas’ disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (kDNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.
Keywords in Portuguese
História
Keywords
Trypanosoma cruzi
Chagas`Disease
Brazil
History
 
DeCS
Trypanosoma cruzi
Doença de Chagas
 
Publisher
Springer Verlag
Citation
LANA, Marta de et al. Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas’ disease described by Carlos Chagas in 1909. Parasitology Research, v. 82, n. 3, p. 257-260, 1996.
DOI
10.1007/s004360050106
ISSN
0932-0113