Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14685
Title: Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
Authors: Goldenberg, S.
Salles, J. M.
Contreras, V. T.
Franco, M. P. Lima
Katzin, A. M.
Colli, W.
Morel, Carlos Medicis
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Niteroi, RJ, Brasil.
Universidad de Carabobo. Valencia, Venezuela.
Universidade Federal Fluminense. Niteroi, RJ, Brasil.
Universidade de São Paulo, Instituto de Química.São Paulo, SP, Brasil.
Universidade de São Paulo, Instituto de Química.São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: The cell-free translation products of polyribosomal and post-polyribosomal mRNAs from the non-infective epimastigotes and the infective metacyclic trypomastigotes of the parasitic protozoan Trypanosoma cruzi were compared by two-dimensional polyacrylamide gel electrophoresis. The result show that although many polypeptides are conserved, quantitative and qualitative differences are observed between both differentiation stages. The results also indicate the existence of post-polyribosomal mRNAs in equilibrium with polyribosomal counterparts. The immunoprecipitation of the in vitro synthesized polypeptides with chagasic human serum and the serum raised against an 85kDa glycoprotein (PZ-WGA), potentially involved in the process of T. cruzi penetration into mammalian cells, shows that while the chagasic serum recognizes the same 72-kDa, 6%kDa and 46-kDa polypeptides in both differentiation stages, the anti-PZWGA serum immunoprecipitates a single 48-kDa polypeptide from in vitro translation products of metacyclic trypomastigotes.
Keywords: T. cruzi
mRNA
Cell-free translation
Surface antigen
Chagas disease
DeCS: Doença de Chagas
Trypanosoma cruzi
RNA Mensageiro
Issue Date: 1985
Publisher: Wiley
Citation: GOLDENBERG, Samuel; et al. Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi. FEBS Letters, v.180, n.2, p.265-270, Jan. 1985.
ISSN: 0014-5793
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
carlos6_morel_etal_IOC_1985.pdf778.64 kBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.