Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14688
Title: Experimental paleoparasitology: identification of Trypanosoma cruzi DNA in desiccated mouse tissue
Authors: Bastos, O. M.
Araújo, A.
Ferreira, L. F.
Santoro, A.
Winckler, P.
Morel, Carlos Medicis
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Niterói, Rj, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil
Abstract: The polymerase chain reaction (PCR) has been used for clinical diagnosis of infectious disease and to research ancient animal and microbiological DNA from a wide range of tissues. PCR was used to study the possibility of Trypanosoma cruzi kinetoplast DNA (kDNA) extraction from experimentally desiccated mouse tissue (heart, skeletal muscle, liver, spleen, and pancreas). The results obtained suggest the application of this technique to T. cruzi detection in archaeological material.
Keywords: Trypanosoma cruzi
Experimental paleoparasitology
Polymerase chain reaction
Kinetoplast DNA
keywords: Trypanosoma cruzi
Paleoparasitologia experimental
Reação em Cadeia da Polimerase
DNA de Cinetoplasto
Issue Date: 1996
Citation: BASTOS, O. M. et al. Experimental paleoparasitology: identification of Trypanosoma cruzi DNA in desiccated mouse tissue. Paleopathology newsletter, v. 94, p. 5-8, 1996.
DOI: PMID: 11613235
ISSN: 0148-4737
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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