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EXPERIMENTAL PALEOPARASITOLOGY: IDENTIFICATION OF TRYPANOSOMA CRUZI DNA IN DESICCATED MOUSE TISSUE
Paleoparasitologia experimental
Reação em Cadeia da Polimerase
DNA de Cinetoplasto
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Abstract
The polymerase chain reaction (PCR) has been used for clinical diagnosis of infectious disease and to research ancient animal and microbiological DNA from a wide range of tissues. PCR was used to study the possibility of Trypanosoma cruzi kinetoplast DNA (kDNA) extraction from experimentally desiccated mouse tissue (heart, skeletal muscle, liver, spleen, and pancreas). The results obtained suggest the application of this technique to T. cruzi detection in archaeological material.
Keywords in Portuguese
Trypanosoma cruziPaleoparasitologia experimental
Reação em Cadeia da Polimerase
DNA de Cinetoplasto
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