Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14695
Title: Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener
Authors: Zingales, Bianca
Pereira, Maria Elizabeth S.
Oliveira, Riva P.
Almeida, Katia A.
Umezawa, Eufrosina S.
Souto, Ricardo P.
Vargas, Nancy
Cano, Maria Isabel
Silveira, José Franco da
Nehme, Nédia S.
Morel, Carlos Medicis
Brener, Zigman
Macedo, Andrea
Affilliation: Universidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
Universidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
Universidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
UNIFESP. Escola Paulista de Medicina. São Paulo, SP, Brasil.
UNIFESP. Escola Paulista de Medicina. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
Abstract: Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. CL Brener was obtained by cloning procedures from bloodstream trypomastigotes isolated from mice infected with the CL strain. the doubling time of CL Brener epimastigotes cultured at 28 degrees C in liver infusion-tryptose (LIT) medium is 58 +/- 13 h. Differentiation to metacyclic forms is induced by incubation of epimastigotes in LIT-20% Grace's medium. Metacyclics give very low parasitemia in mice, contrary to what is observed for blood forms which promote 100% mortality of the animals with inocula of 5 x 10(3) parasites. CL Brener blood forms are highly susceptible to nifurtimox, benznidazole and ketoconazole. Allopurinol is inefficient in the treatment of mice experimental infection. the clone infects mammalian cultured cells and performs the complete intracellular cycle at 33 and 37 degrees C. the molecular typing of CL Brener has been done by isoenzymatic profiles; sequencing of a 24S alpha ribosomal RNA gene domain and by schizodeme, randomly amplified polymorphic DNA and DNA fingerprinting analyses. for each typing approach the patterns obtained do not change after prolonged parasite subcultivation in LIT medium (up to 100 generations). the stability of the molecular karyotype of the clone was also confirmed.
Keywords: Trypanosoma cruz
Genome project
Reference organism
Molecular typing
Biological characteristics
DeCS: Trypanosoma cruzi
Projeto Genoma
Tipagem Molecular
Características biológicas
Issue Date: 1997
Publisher: Elsevier
Citation: ZINGALES, Bianca et al. Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone Cl Brener. Acta Tropica, v. 68, p. 159-173, 1997.
DOI: 10.1016/S0001-706X(97)00088-0
ISSN: 0001-706X
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos
IOC - Artigos de Periódicos

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