Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14699
Title: Pcr-based diagnosis for chagas disease in Bolivian children living in a active transmission area: comparison with conventional serological and parasitological diagnosis
Authors: Wincker, P.
Telleria, J
Bosseno, M. F.
Cardoso, M. A.
Marques, P.
Yaksic, N.
Aznar, C.
Liegeard, P.
Hontebeyrie, M.
Noireau, F.
Morel, Carlos Medicis
Breniere, S. F.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil / Faculté de Medicine. Laboratoire Génome des Parasites, Parasitologie. Montpellier, France.
UR41, UMR ORSTOM/CNRS 9926 "Génetique moléculaire des Parasites et des Vecteurs". La Paz, Bolivia.
UR41, UMR ORSTOM/CNRS 9926 "Génetique moléculaire des Parasites et des Vecteurs". La Paz, Bolivia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Universidad Mayor de San Andres. IBBA. La Paz, Bolivia.
Institut Pasteur, Départment d`Immunologie et Centre de Biologie Médicale. Paris, France.
Institut Pasteur, Départment d`Immunologie et Centre de Biologie Médicale. Paris, France.
UR41 ORSTOM. La Paz, Bolivia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
UR41, UMR ORSTOM/CNRS 9926 "Génetique moléculaire des Parasites et des Vecteurs". La Paz, Bolivia.
Abstract: A large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnostic methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buffy coat examination, 106 were detected by PCR (sensitivity: 93.8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, IgG-, IgM-, and buffy coat-negative) only 1 case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections.
Keywords: Trypanosoma cruzi
Chagas`Disease
PCR diagnosis
Serological diagnosis
DeCS: Trypanosoma cruzi
Doença de Chagas
Reação em Cadeia da Polimerase
Diagnóstico
Sorodiagnóstico
Issue Date: 1997
Publisher: Cambridge University Press
Citation: WINCKER, P. et al. Pcr-based diagnosis for chagas disease in Bolivian children living in a active transmission area: comparison with conventional serological and parasitological diagnosis. Parasitology, v. 114, p. 367-373, 1997.
DOI: 10.1017/S0031182096008554
ISSN: 0031-1820
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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