| Author | Fernandes, O. | |
| Author | Mangia, R. H. | |
| Author | Lisboa, C. V. | |
| Author | Pinho, A. P. | |
| Author | Morel, Carlos Medicis | |
| Author | Zingales, B. | |
| Author | Campbell, D. A. | |
| Author | Jansen, A. M. | |
| Access date | 2016-06-30T13:17:06Z | pt_BR |
| Access date | 2016-07-07T12:22:54Z | |
| Available date | 2016-06-30T13:17:06Z | pt_BR |
| Available date | 2016-07-07T12:22:54Z | |
| Document date | 1999 | |
| Citation | FERNANDES, O. et al. The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the non-transcribed spacer of the mini-exon gene. Parasitology, v. 118, n. 2, p. 161-166, fev. 1999. | pt_BR |
| ISSN | 0031-1820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14702 | |
| Language | eng | pt_BR |
| Publisher | Cambridge University Press | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Ciclo silvestre | pt_BR |
| Subject in Portuguese | Rio de Janeiro | pt_BR |
| Title | The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene | pt_BR |
| Type | Article | |
| DOI | 10.1017/s0031182098003709 | pt_BR |
| Abstract | American trypanosamiasis occurs in nature as a sylvatic cycle, where Trypanosoma cruzi interacts with wild triatomines
and mammalian reservoirs, such as marsupials, rodents, armadillos and other animals. Due to difficulties in trying to
isolate T. cruzi stocks from the sylvatic cycle, very few studies have been performed in order to understand the parasite
infection in natural environments. Traditionally T. cruzi has been considered to be composed of a highly heterogeneous
population of parasites. In contrast, the mini-exon and the 24Sα rRNA gene loci have shown that T. cruzi stocks can be
clustered in 2 major phylogenetic groups: lineage 1 and lineage 2. In this report, 68 recently isolated T. cruzi samples from
the sylvatic cycle belonging to different geographical areas in Rio de Janeiro, Brazil, have been typed based on a variable
spot in the non-transcribed spacer of the mini-exon gene. Eight isolates were from triatomines, 26 stocks were from
golden-lion tamarins, 31 from opossums, 2 from rodents and 1 from a three-toed sloth. Thirty (44% – 30}68) isolates were
typed as lineage 1, while 36 (53% – 36}68) isolates were typed as lineage 2. Two opossums presented mixed infection.
Therefore, 3% (2}68) of the isolates were typed as lineage 1lineage 2. Using these geographical regions as models of
sylvatic environments, it was observed that 96% of the Didelphis marsupialis were infected by lineage 2 isolates, while all
26 golden-lion tamarins were infected by lineage 1. The results show preferential association of the 2 lineages of T. cruzi
with different hosts, composing the complexity of the sylvatic cycle. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Mini-exon gene | pt_BR |
| Subject | Sylvatic cycle | pt_BR |
| Subject | Didelphis marsupialis | pt_BR |
| Subject | Leontophitecus rosalia | pt_BR |
| Subject | Rio de Janeiro | pt_BR |
