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ELECTROPHORETIC ANALYSIS OF ENDONUCLEASE-GENERATED FRAGMENTS OF K-DNA, OF ESTERASE ISOENZYMES, AND OF SURFACE PROTEINS AS AIDS FOR SPECIES IDENTIFICATION OF INSECT TRYPANOSOMATIDS
Aids
Endonuclease-Generated Fragments of k-DNA
Electrophoretic Analysis
Esterase Isoenzymes
Affilliation
Escola Paulista de Medicina. Departamento de Micro, Imuno e Parasitologia. São Paulo, SP, Brasil.
Escola Paulista de Medicina. Departamento de Micro, Imuno e Parasitologia. São Paulo, SP, Brasil.
Escola Paulista de Medicina. Departamento de Micro, Imuno e Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Escola Paulista de Medicina. Departamento de Micro, Imuno e Parasitologia. São Paulo, SP, Brasil.
Escola Paulista de Medicina. Departamento de Micro, Imuno e Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Abstract
In order to verify the applicability of biochemical methods for species identification of Trypanosomatidae, 13 species
of monoxenic trypanosomatids plus the heteroxenous Trypanusuma cruzi were comparatively analyzed by three different biochemical
methods. Insect trypanosomatids examined were: Crithidia acanthocephali, C. fasciculata (three varieties), C. luciliae luciliae, C.
luciliae thermophila, C. deanei, C. oncopelti, Herpetomonas muscarum muscarum, H. megaseliae, H. samuelpessoai, H. mariadeanei,
Leptomonas seymouri, L. collosoma, L. samueli. and BIasiocrithidia culicis. Also included in the survey were aposymbiotic strains
of C. dranei and C. oncopelti. Methods used were: electrophoretic profiling of endonuclease-generated fragments of k-DNA, esterase
isoenzymes profiling, and polyacrylamide-gel electrophoresis (SDS-PAGE) of radioiodinated cell surface proteins. Interspecific but not
intraspecific differences were detected by all three methods among the 13 monoxenic species examined. Thus, it is concluded that
these methods can be successfully used, in addition to classical criteria, for species identification of insect trypanosomatids.
Keywords in Portuguese
Análise eletroforéticaKeywords
Insect TrypanosomatidsAids
Endonuclease-Generated Fragments of k-DNA
Electrophoretic Analysis
Esterase Isoenzymes
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