Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14780
Title: Proteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease
Authors: Ferreira, Roberto Rodrigues
Souza, Elen Mello de
Oliveira, Fabiane Loiola de
Ferrão, Patrícia Mello
Gomes, Leonardo Henrique Ferreira
LIma, Leila Mendonça
Batista, Marcelo Meuser
Bailly, Sabine
Feige, Jean Jacques
Araujo-Jorge, Tania C.
Waghabi, Mariana Caldas
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
INSERM. U1036, Grenoble F-38000, France / University Grenoble-Alpes. Grenoble F-38000, France / CEA, DSV, iRTSV, Laboratory of Biology of Cancer and Infection. Grenoble F-38000, France.
INSERM. U1036, Grenoble F-38000, France / University Grenoble-Alpes. Grenoble F-38000, France / CEA, DSV, iRTSV, Laboratory of Biology of Cancer and Infection. Grenoble F-38000, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Abstract: Studies developed by our group in the last years have shown the involvement of TGF-β in acute and chronic Chagas heart disease, with elevated plasma levels and activated TGF-β cell signaling pathway as remarkable features of patients in the advanced stages of this disease, when high levels of cardiac fibrosis is present. Imbalance in synthesis and degradation of extracellular matrix components is the basis of pathological fibrosis and TGF-β is considered as one of the key regulators of this process. In the present study, we investigated the activity of the TGF-β signaling pathway, including receptors and signaling proteins activation in the heart of animals experimentally infected with Trypanosoma cruzi during the period that mimics the acute phase of Chagas disease. We observed that T. cruzi—infected animals presented increased expression of TGF-β receptors. Overexpression of receptors was followed by an increased phosphorylation of Smad2/3, p38 and ERK. Furthermore, we correlated these activities with cellular factors involved in the fibrotic process induced by TGF-β. We observed that the expression of collagen I, fibronectin and CTGF were increased in the heart of infected animals on day 15 post-infection. Correlated with the increased TGF-β activity in the heart, we found that serum levels of total TGF-β were significantly higher during acute infection. Taken together, our data suggest that the commitment of the heart associates with increased activity of TGF-β pathway and expression of its main components. Our results, confirm the importance of this cytokine in the development and maintenance of cardiac damage caused by T. cruzi infection.
Keywords: Trypanosoma cruzi
TGF-β
Infection
Chagas Disease
Fibrosis
keywords: Trypanosoma cruzi
Doença de Chagas
Infecção
Fibrose
Issue Date: 2016
Publisher: ScienceDirect
Citation: FERREIRA, Roberto Rodrigues; et al. Proteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease. Immunobiology, v.221, n.5, p.587-594, May 2016.
DOI: 10.1016/j.imbio.2016.01.009
ISSN: 0171-2985
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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