Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14908
Title: Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
Authors: González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Bussy, Rodrigo Fernández
Spinelli, Silvana Virginia
D`Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Almeida, Vinícius Cotta de
Villar, Silvina Raquel
Pérez, Ana Rosa
Affilliation: Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
Abstract: The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (α chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease.
Keywords: Trypanosoma cruzi
Thymic
Regulatory T cells
Infection
keywords: Trypanosoma cruzi
Células T reguladoras
Infecção
Timo
Issue Date: 2016
Publisher: Public Library of Science
Citation: GONZÁLEZ, Florencia Belén; et al. Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. PLoS Negl Trop Dis., v.10, n.1, e0004285, 21p, Jan. 2016.
DOI: 10.1371/journal.pntd.0004285
ISSN: 1935-2727
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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