Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14944
Title: Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax
Authors: Costa, Joana D’Arc Neves
Zanchi, Fernando Berton
Rodrigues, Francisco Lurdevanhe da Silva
Honda, Eduardo Rezende
Katsuragawa, Tony Hiroschi
Pereira, Dhélio Batista
Taborda, Roger Lafontaine Mesquita
Tada, Mauro Shugiro
Ferreira, Ricardo de Godoi Mattos
Pereira-da-Silva, Luiz Hildebrando
Affilliation: Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brasil.
Abstract: The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfClag9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections.
Keywords: Malaria
Immunity
Amazon
Cross-reaction
Issue Date: 2013
Publisher: Fundação Oswaldo Cruz
Citation: COSTA, Joana D’Arc Neves et al. Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 98-105, Feb. 2013.
DOI: 10.1590/S0074-02762013000100016
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos

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