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2030-01-01
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- IOC - Artigos de Periódicos [12828]
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DETECTION AND QUANTIFICATION OF CLASSIC AND EMERGING VIRUSES BY SKIMMED-MILK FLOCCULATION AND PCR IN RIVER WATER FROM TWO GEOGRAPHICAL AREAS
Adenovírus
Poliomavirus
Poliomavírus das Células de Merkel
Norovírus
Método de concentração
Indicador viral
Polyomavirus
Merkel cell polyomavirus
Klassevirus
Asfarvirus-like virus
Rotavirus
Norovirus
Adenovirus
River water
Concentration method
Viral indicator
Author
Affilliation
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
University of Buenos Aires. Faculty of Pharmacy and Biochemistry. Laboratory of Virology. Buenos Aires, Argentina.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
University of Buenos Aires. Faculty of Pharmacy and Biochemistry. Laboratory of Virology. Buenos Aires, Argentina.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Barcelona. Faculty of Biology. Department of Microbiology. Barcelona, Spain.
Abstract
Molecular techniques and virus concentration methods have shown that previously unknown viruses are shed by humans and animals, and may be transmitted by sewage-contaminated water. In the present study, 10-L river-water samples from urban areas in Barcelona, Spain and Rio Janeiro, Brazil, have been analyzed to evaluate the viral dissemination of human viruses, validating also a low-cost concentration method for virus quantification in fresh water. Three viral groups were analyzed: (i) recently reported viruses, klassevirus (KV), asfarvirus-like virus (ASFLV), and the polyomaviruses Merkel cell (MCPyV), KI (KIPyV) and WU (WUPyV); (ii) the gastroenteritis agents noroviruses (NoV) and rotaviruses (RV); and (iii) the human fecal viral indicators in water, human adenoviruses (HAdV) and JC polyomaviruses (JCPyV). Virus detection was based on nested and quantitative PCR assays. For KV and ASFLV, nested PCR assays were developed for the present study. The method applied for virus concentration in fresh water samples is a one-step procedure based on a skimmed-milk flocculation procedure described previously for seawater. Using spiked river water samples, inter- and intra-laboratory assays showed a viral recovery rate of about 50% (20-95%) for HAdV, JCPyV, NoV and RV with a coefficient of variation ≤ 50%. HAdV and JCPyV were detected in 100% (12/12) of the river samples from Barcelona and Rio de Janeiro. Moreover, NoV GGII was detected in 83% (5/6) and MCPyV in 50% (3/6) of the samples from Barcelona, whereas none of the other viruses tested were detected. NoV GGII was detected in 33% (2/6), KV in 33% (2/6), ASFLV in 17% (1/6) and MCPyV in 50% (3/6) of the samples from Rio de Janeiro, whereas KIPyV and WUPyV were not detected. RV were only analyzed in Rio de Janeiro and resulted positive in 67% (4/6) of the samples. The procedure applied here to river water represents a useful, straightforward and cost-effective method that could be applied in routine water quality testing. The results of the assays expand our understanding of the global distribution of the viral pathogens studied here and their persistence in the environment.
Keywords in Portuguese
RotavírusAdenovírus
Poliomavirus
Poliomavírus das Células de Merkel
Norovírus
Método de concentração
Indicador viral
Keywords
Emerging virusPolyomavirus
Merkel cell polyomavirus
Klassevirus
Asfarvirus-like virus
Rotavirus
Norovirus
Adenovirus
River water
Concentration method
Viral indicator
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