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ENAMEL FORMATION GENES INFLUENCE ENAMEL MICROHARDNESS BEFORE AND AFTER CARIOGENIC CHALLENGE
Shimizu, Takehiko et al. | Date Issued: 2012
Author
Shimizu, Takehiko
Ho, Bao
Deeley, Kathleen
Briseño-Ruiz, Jessica
Faraco, Italo M.
Schupack, Brett I.
Brancher, João A.
Pecharki, Giovana D.
Küchler, Erika C.
Tannure, Patricia N.
Lips, Andrea
Vieira, Thays C. S.
Patir, Asli
Yildirim, Mine
Poletta, Fernando A.
Mereb, Juan C.
Resick, Judith M.
Brandon, Carla A.
Orioli, Iêda M.
Castilla, Eduardo E.
Marazita, Mary L.
Seymen, Figen
Costa, Marcelo C.
Granjeiro, José M.
Trevilatto, Paula C.
Vieira, Alexandre R.
Affilliation
Nihon University of Dentistry. 1 Department of Pediatric Dentistry. Matsudo, Chiba, Japan.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
Pontifícia Universidade Católica do Paraná (PUCPR). Centro de Ciências Biológicas e da Saúde. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná (PUCPR). Centro de Ciências Biológicas e da Saúde. Curitiba, PR, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Unidade de Pesquisa Clínica. Nterói, RJ, Brasil.
Universidade Veiga de Almeida. Disciplina de Odontologia Pediátrica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Odontologia Pediátrica e Ortodôntica. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Unidade de Pesquisa Clínica. Nterói, RJ, Brasil.
Istanbul Medipol University. Department of Pedodontics. Istanbul, Turkey.
Istanbul Medipol University. Faculty of Dentistry. Department of Periodontics. Istanbul, Turkey.
Latin American Collaborative Study of Congenital Malformations (ECLAMC). Center for Medical Education and Clinical Research (CEMIC). Buenos Aires, Argentina.
Latin American Collaborative Study of Congenital Malformations (ECLAMC). Hospital de Area El Bolsón, Río Negro, Argentina.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA.
Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biologia. Departamento de Genética. Latin American Collaborative Study of Congenital Malformations (ECLAMC). National Institute of Population Medical Genetics (INAGEMP-CNPq). Rio de Janeiro, RJ, Brasil.
Latin American Collaborative Study of Congenital Malformations (ECLAMC). Center for Medical Education and Clinical Research (CEMIC). Buenos Aires, Argentina / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Latin American Collaborative Study of Congenital Malformations (ECLAMC). National Institute of Population Medical Genetics (INAGEMP-CNPq). Rio de janeiro, RJ, Brasil.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA / , University of Pittsburgh. Center for Craniofacial and Dental Genetics. Department of Human Genetics, and Clinical and Translational Science. Pittsburg, Pensylvania, USA.
Istanbul University. Faculty of Dentistry. Department of Periodontics. Istambul, Turkey.
Universidade Federal do Rio de Janeiro. Departamento de Odontologia Pediátrica e Ortodôntica. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Unidade de Pesquisa Clínica. Nterói, RJ, Brasil.
Pontifícia Universidade Católica do Paraná (PUCPR). Centro de Ciências Biológicas e da Saúde. Curitiba, PR, Brasil.
University of Pittsburgh. Department of Oral Biology. Pittsburgh, PA, USA. / University of Pittsburgh. Center for Craniofacial and Dental Genetics. Department of Pediatric Dentistry, School of Dental Medicine. Clinical and Translational Science. Pittsburgh, PA, USA.
Abstract
There is evidence for a genetic component in caries susceptibility, and studies in humans have suggested that variation in enamel formation genes may contribute to caries. For the present study, we used DNA samples collected from 1,831 individuals from various population data sets. Single nucleotide polymorphism markers were genotyped in selected genes (ameloblastin, amelogenin, enamelin, tuftelin, and tuftelin interacting protein 11) that influence enamel formation. Allele and genotype frequencies were compared between groups with distinct caries experience. Associations with caries experience can be detected but they are not necessarily replicated in all population groups and the most expressive results was for a marker in AMELX (p=0.0007). To help interpret these results, we evaluated if enamel microhardness changes under simulated cariogenic challenges are associated with genetic variations in these same genes. After creating an artificial caries lesion, associations could be seen between genetic variation in TUFT1 (p=0.006) and TUIP11 (p=0.0006) with enamel microhardness. Our results suggest that the influence of genetic variation of enamel formation genes may influence the dynamic interactions between the enamel surface and the oral cavity.
Keywords in Portuguese
Cárie Dentária
Esmalte Dentário
Cariogênicos
Genes formação do esmalte
Cavidade oral
 
Keywords
Caries
Enamel formation genes
Cariogenic Challenge
Enamel Microhardness
Oral cavity
 
Publisher
BioMed Central
Citation
SHIMIZU, Takehiko; et al. Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge. Plos One, v.7, n.9, e45022, 9p, Sept. 2012.
DOI
10.1371/journal.pone.0045022
ISSN
1932-6203