Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/15311
Title: Apoptosis in a canine model of acute Chagasic myocarditis
Authors: Zhang, J
Andrade, Zilton de Araújo
Yu, Z. X
Andrade, Sonia Gumes
Takeda, K
Sadigursky, Moysés
Ferrans, V. J
Affilliation: National Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USA
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Food and Drug Administration. Division of Applied Pharmacology Research. Laurel, MD, USA
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
National Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USA
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Food and Drug Administration. Division of Applied Pharmacology Research. Laurel, MD, USA
Abstract: Histologic, ultrastructural and nick end labeling studies were made to evaluate the occurrence of apoptosis in the hearts of dogs with acute myocarditis due to experimental infection with T. cruzi. The best results for the detection of apoptosis by nick end labeling were obtained by a method combining the use of terminal deoxynucleotidyl transferase, CoCl2 and fluorescein-conjugated deoxyuridine triphosphate, followed by counterstaining of DNA with 4'6-diamidino-2-phenylindole (DAPI) and examination by laser scanning confocal fluorescence microscopy. Apoptosis was found in: (1) cardiac myocytes; (2) endothelial cells of capillaries and venules: (3) immune effector cells, including macrophages, interstitial dendritic cells (antigen-presenting cells) and granular and agranular lymphocytes, and (4) intra- and extracellular forms of T. cruzi. The apoptosis in myocytes and endothelial cells affected cells that were not infected by T. cruzi and was probably caused by the release of toxic mediators of inflammation. The apoptosis of immune effector cells could be related either to the subsidence of inflammation or to modulation (and even failure) of the immune response. The finding of apoptosis in T. cruzi confirms the results of other studies showing that this phenomenon occurs during the differentiation of trypomastigotes in vitro. Thus, apoptosis constitutes an important and multifactorial event in the pathogenesis of acute Chagasic myocarditis.
Keywords: Trypanosoma cruzi
Apoptosis
Acute myocarditis
Dog
Nick end labeling
Electron microscopy
Issue Date: 1999
Publisher: Elsevier
Citation: ZHANG, J. et al. Apoptosis in a canine model of acute Chagasic myocarditis. Journal of Molecular and Cellular Cardiology, v. 31, p. 581–596, 1999.
DOI: 10.1006/jmcc.1998.0893
ISSN: 0022-2828
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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