Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/15871
Title: The Brazilian network for HIV-1 genotyping external quality control assurance programme
Authors: Souza, Denise C. F.
Sucupira, Maria Cecilia A.
Brindeiro, Rodrigo M.
Fernandez, José Carlos C.
Sabino, Ester C.
Inocencio, Lilian A.
Diaz, Ricardo S.
Affilliation: Ministério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil .
Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil .
Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Pro-Sangue. Hemocentro de São Paulo. São Paulo, SP, Brasil.
Ministério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil .
Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil .
Abstract: The Brazilian network for genotyping is composed of 21 laboratories that perform and analyze genotyping tests for all HIV-infected patients within the public system, performing approximately 25,000 tests per year. We assessed the interlaboratory and intralaboratory reproducibility of genotyping systems by creating and implementing a local external quality control evaluation. Plasma samples from HIV-1-infected individuals (with low and intermediate viral loads) or RNA viral constructs with specific mutations were used. This evaluation included analyses of sensitivity and specificity of the tests based on qualitative and quantitative criteria, which scored laboratory performance on a 100-point system. Five evaluations were performed from 2003 to 2008, with 64% of laboratories scoring over 80 points in 2003, 81% doing so in 2005, 56% in 2006, 91% in 2007, and 90% in 2008 (Kruskal-Wallis, p = 0.003). Increased performance was aided by retraining laboratories that had specific deficiencies. The results emphasize the importance of investing in laboratory training and interpretation of DNA sequencing results, especially in developing countries where public (or scarce) resources are used to manage the AIDS epidemic.
Keywords: Brazilian Network
HIV-1 Genotyping
HIV-infected patients
keywords: HIV-1
Coinfecção por HIV
Rede Brasileira
Genotipagem HIV-1
Issue Date: 2011
Publisher: BioMed Central
Citation: SOUZA, Denise C. F. et al. The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme. Journal of the International AIDS Society, v.14:45, 6p, 2011.
DOI: 10.1186/1758-2652-14-45
ISSN: 1758-2652
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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