Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/15897
Title: Oral exposure to Phytomonas serpens attenuates thrombocytopenia and leukopenia during acute infection with Trypanosoma cruzi
Authors: Silva, Rosiane Valeriano da
Malvezi, Aparecida Donizette
Augusto, Leonardo da Silva
Kian, Danielle
Tatakihara, Vera Lúcia Hideko
Yamauchi, Lucy Megumi
Yamada-Ogatta, Sueli Fumie
Rizzo, Luiz V.
Schenkman, Sergio
Pinge-Filho, Phileno
Affilliation: Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.
Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.
Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.
Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.
Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.
Instituto Israelita de Ensino e Pesquisa Albert Einstein. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract: Mice infected with Trypanosoma cruzi, the agent of Chagas disease, rapidly develop anemia and thrombocytopenia. These effects are partially promoted by the parasite trans-sialidase (TS), which is shed in the blood and depletes sialic acid from the platelets, inducing accelerated platelet clearance and causing thrombocytopenia during the acute phase of disease. Here, we demonstrate that oral immunization of C57BL/6 mice with Phytomonas serpens, a phytoflagellate parasite that shares common antigens with T. cruzi but has no TS activity, reduces parasite burden and prevents thrombocytopenia and leukopenia. Immunization also reduces platelet loss after intraperitoneal injection of TS. In addition, passive transfer of immune sera raised in mice against P. serpens prevented platelet clearance. Thus, oral exposure to P. serpens attenuates the progression of thrombocytopenia induced by TS from T. cruzi. These findings are not only important for the understanding of the pathogenesis of T. cruzi infection but also for developing novel approaches of intervention in Chagas disease.
Keywords: Trypanosoma cruzi
Chagas disease
parasite trans-sialidase
Kinetoplastida pathogens
DeCS: Doença de Chagas
Parasitos
Interações Hospedeiro-Patógeno
Trombocitopenia
Leucopenia
Issue Date: 2013
Publisher: Kerry Kroffe
Citation: SILVA, Rosiane V. da et al. Oral exposure to Phytomonas serpens attenuates thrombocytopenia and leukopenia during acute infection with Trypanosoma cruzi. PLoS ONE, v. 8, n. 7, p.1-9, 2013.
DOI: 10.1371/journal.pone.0068299
ISSN: 1932-6203
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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