| Author | Macêdo, Regina C. L. de | |
| Author | Rosado, Alexandre S. | |
| Author | Mota, Fabio F. da | |
| Author | Cavalcante, Maria A. S. | |
| Author | Eulálio, Kelsen D. | |
| Author | D. Filho, Antônio | |
| Author | Martins, Liline M. S. | |
| Author | Lazéra, Márcia S. | |
| Author | Wanke, Bodo | |
| Access date | 2016-09-29T11:37:48Z | |
| Available date | 2016-09-29T11:37:48Z | |
| Document date | 2011 | |
| Citation | MACÊDO, Regina C. L. de et al. Molecular identification of Coccidioides spp. in soil samples from Brazil. BMC Microbiology, v. 11, n. 108, p. 1-9, 2011. | pt_BR |
| ISSN | 1471-2180 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15993 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Região Nordeste | pt_BR |
| Subject in Portuguese | Semi árido | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Coccidioidomicose | pt_BR |
| Subject in Portuguese | Coccidioides | pt_BR |
| Title | Molecular identification of Coccidioides spp. in soil samples from Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1186/1471-2180-11-108 | |
| Abstract | Background: Since 1991 several outbreaks of acute coccidioidomycosis (CM) were diagnosed in the semi-arid
Northeast of Brazil, mainly related to disturbance of armadillo burrows caused by hunters while digging them for
the capture of these animals. This activity causes dust contaminated with arthroconidia of Coccidioides posadasii,
which, once inhaled, cause the mycosis. We report on the identification of C. posadasii in soil samples related to
outbreaks of CM.
Results: Twenty four soil samples had their DNA extracted and subsequently submitted to a semi-nested PCR
technique using specific primers. While only 6 (25%) soil samples were positive for C. posadasii by mice inoculation,
all (100%) were positive by the molecular tool.
Conclusion: This methodology represents a simple, sensitive and specific molecular technique to determine the
environmental distribution of Coccidioides spp. in endemic areas, but cannot distinguish the species. Moreover, it
may be useful to identify culture isolates. Key-words: 1. Coccidioidomycosis. 2. Coccidioides spp. 3. C. posadasii.
4. Semi-arid. 5. Semi-nested PCR. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Teresina, PI, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Teresina, PI, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Coccidioidomycosis | pt_BR |
| Subject | Northeast of Brazil | pt_BR |
| Subject | Coccidioides spp | pt_BR |
| Subject | C. posadasii | pt_BR |
| Subject | Semi-arid | pt_BR |
| Subject | Semi-nested PCR | pt_BR |
