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MOLECULAR IDENTIFICATION OF COCCIDIOIDES SPP. IN SOIL SAMPLES FROM BRAZIL
Macêdo, Regina C. L. de et al. | Date Issued: 2011
Author
Macêdo, Regina C. L. de
Rosado, Alexandre S.
Mota, Fabio F. da
Cavalcante, Maria A. S.
Eulálio, Kelsen D.
D. Filho, Antônio
Martins, Liline M. S.
Lazéra, Márcia S.
Wanke, Bodo
Affilliation
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.
Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil.
Universidade Federal do Piauí. Teresina, PI, Brasil.
Universidade Federal do Piauí. Teresina, PI, Brasil.
Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Abstract
Background: Since 1991 several outbreaks of acute coccidioidomycosis (CM) were diagnosed in the semi-arid Northeast of Brazil, mainly related to disturbance of armadillo burrows caused by hunters while digging them for the capture of these animals. This activity causes dust contaminated with arthroconidia of Coccidioides posadasii, which, once inhaled, cause the mycosis. We report on the identification of C. posadasii in soil samples related to outbreaks of CM. Results: Twenty four soil samples had their DNA extracted and subsequently submitted to a semi-nested PCR technique using specific primers. While only 6 (25%) soil samples were positive for C. posadasii by mice inoculation, all (100%) were positive by the molecular tool. Conclusion: This methodology represents a simple, sensitive and specific molecular technique to determine the environmental distribution of Coccidioides spp. in endemic areas, but cannot distinguish the species. Moreover, it may be useful to identify culture isolates. Key-words: 1. Coccidioidomycosis. 2. Coccidioides spp. 3. C. posadasii. 4. Semi-arid. 5. Semi-nested PCR.
Keywords in Portuguese
Região Nordeste
Semi árido
Brasil
Reação em Cadeia da Polimerase
Coccidioidomicose
Coccidioides
 
Keywords
Coccidioidomycosis
Northeast of Brazil
Coccidioides spp
C. posadasii
Semi-arid
Semi-nested PCR
 
Publisher
BioMed Central
Citation
MACÊDO, Regina C. L. de et al. Molecular identification of Coccidioides spp. in soil samples from Brazil. BMC Microbiology, v. 11, n. 108, p. 1-9, 2011.
DOI
10.1186/1471-2180-11-108
ISSN
1471-2180