Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16098
Title: IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis
Authors: Jesus, Laura Néspoli Nassar Pansini de
Tonini, Aline de Castro Zacche
Barros, Geisa Baptista
Reis, Jordana Grazziela Alves Coelho dos
Béla, Samantha Ribeiro
Antonelli, Lis Ribeiro do Valle
Machado, Anderson Silva
Carneiro, Ana Carolina Aguiar Vasconcelos
Andrade, Gláucia Manzan Queiroz
Santos, Daniel Vitor Vasconcelos
Januário, José Nélio
Carvalho, Andréa Teixeira de
Vitor, Ricardo Wagner Almeida
Ferro, Eloísa Amália Vieira
Mineo, José Roberto
Oliveira, Lilian Maria Garcia Bahia
Martins Filho, Olindo Assis
Lemos, Elenice Moreira
Affilliation: Universidade Federal do Espírito Santo. Vitória, ES, Brasil
Universidade Federal do Espírito Santo. Vitória, ES, Brasil
Universidade Federal do Espírito Santo. Vitória, ES, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Núcleo de Ações e pesquisa em Apoio Diagnóstico. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil
Universidade Federal de Uberlândia. Uberlândia, MG, Brasil
Universidade Federal de Uberlândia. Uberlândia, MG, Brasil
Universidade Federal do Rio de Janeiro. Macaé, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal do Espírito Santo. Vitória, ES, Brasil
Abstract: This study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were observed when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 were closely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8(+) T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4(+) and CD8(+) T-cells but positively correlated with IL-10(+) B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.
Keywords: Cytokines
IgA
IgG1
Ocular congenital toxoplasmosis
T. gondii
keywords: IgA
IgG1
Issue Date: 2016
Publisher: Elsevier Scientific Pub. Co
Citation: JESUS, Laura Néspoli Nassar Pansini de et al. IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis. J Immunol Methods. , vol. 428, p. 1-8, 2016.
DOI: 10.1016/j.jim.2015.11.004
ISSN: 0022-1759
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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