Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16130
Title: Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing
Authors: Ferreira, Roberta Carvalho
Kessler, Rafael Luis
Lorenzo, Marcelo Gustavo
Paim, Rafaela Magalhães Macedo
Ferreira, Luciana de Lima
Probst, Christian Macagnan
Silva, Juliana Alves
Guarneri, ALessandra Aparecida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brasil.
Abstract: Trypanosoma cruzi, the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate the development and differentiation of T. cruzi inside the intestinal tract of Rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. Approximately 20% of the ingested parasites was found in the insect anterior midgut (AM) 3 h after feeding. Interestingly, a significant reduction (80%) in the numbers of trypomastigotes was observed after 24 h of infection suggesting that parasites were killed in the AM. Moreover, few parasites were found in that intestinal portion after 96 h of infection. The evaluation of the numbers of parasites in the posterior midgut (PM) at the same periods showed a reduced parasite load, indicating that parasites were not moving from the AM. Additionally, incubation of blood trypomastigotes with extracts from R. prolixus AMs revealed that components of this tissue could induce significant death of T. cruzi. Finally, we observed that differentiation from trypomastigotes to epimastigotes is not completed in the AM; instead we suggest that trypomastigotes change to intermediary forms before their migration to the PM, where differentiation to epimastigotes takes place. The present work clarifies controversial points concerning T. cruzi development in insect vector, showing that parasite suffers a drastic decrease in population size before epimastigonesis accomplishment in PM.
Keywords: Trypanosoma Cruzi
Rhodnius prolixus
Epimastigogenesis
Trypanosome
Chagas disease
keywords: Trypanosoma cruzi
Rhodnius prolixus
Issue Date: 2016
Publisher: Cambridge University Press
Citation: FERREIRA, Roberta Carvalho et al. Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing. Parasitology, v. 143, n. 4, p. 434-443, 2016.
DOI: 10.1017/S0031182015001857
ISSN: 0031-1820
Copyright: restricted access
Appears in Collections:PR - ICC - Artigos de Periódicos
MG - IRR - Artigos de Periódicos

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