Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16190
Title: Genotyping did not evidence any contribution of Mycobacterium bovis to human tuberculosis in Brazil
Authors: Rocha, Adalgiza
Elias, Atina R.
Sobral, Luciana F.
Soares, Diego F.
Santos, Alexandre C.
Marsico, Ana-Grazia
Hacker, Mariana A.
Caldas, Paulo C.
Parente, Luiz C.
Silva, Marcio R.
Fonseca, Leila
Suffys, Philip
Boéchat, Neio
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Ministério da Saúde. Fundação Oswaldo Cruz. Centro de Referência Nacional em Tuberculose Prof. Hélio Fraga. Rio de Janeiro, RJ, Brasil.
Clínica de Tuberculose Augusto Guimarães, Centro de Referencia Regional em Tuberculose. Program de Controle de Tuberculose. Campos, RJ, Brasil;.
EMBRAPA. Divisão de Saúde Animal. Juiz de Fora, MG, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Medicina Interna. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Pesquisa Multidisciplinar. Rio de Janeiro, RJ, Brasil.
Abstract: The contribution of Mycobacterium bovis to the global burden of tuberculosis (TB) in man is likely to be underestimated due to its dysgonic growth characteristics and because of the absence of pyruvate in most used media is disadvantageous for its primary isolation. In Brazil Mycobacterium culture, identification and susceptibility tests are performed only in TB reference centers, usually for selected cases. Moreover, solid, egg-based, glycerol-containing (without pyruvate supplementation) Löwenstein-Jensen (L-J) or Ogawa media are routinely used, unfavouring M. bovis isolation. To determine the importance of M. bovis as a public health threat in Brazil we investigated 3046 suspected TB patients inoculating their clinical samples onto routine L-J and L-J pyruvate enriched media. A total of 1796 specimens were culture positive for Mycobacterium spp. and 702 TB cases were confirmed. Surprisingly we did not detect one single case of M. bovis in the resulting collection of 1674 isolates recovered from M. bovis favourable medium analyzed by conventional and molecular speciation methods. Also, bacillary DNA present on 454 sputum smears from 223 TB patients were OxyR genotyped and none was recognized as M. bovis. Our data indicate that M. bovis importance on the burden of human TB in Brazil is marginal.
Keywords: Tuberculosis
Mycobacterium tuberculosis complex
M. bovis
M. tuberculosis
Spoligotyping
Brasil
keywords: Tuberculose
Mycobacterium bovis
Mycobacterium tuberculosis
Brasil
Issue Date: 2011
Publisher: Churchill Livingstone
Citation: ROCHA, Adalgiza Rocha; et al. Genotyping did not evidence any contribution of Mycobacterium bovis to human tuberculosis in Brazil. Tuberculosis, v.91, p.14-21, 2011.
DOI: 10.1016/j.tube.2010.10.003
ISSN: 1472-9792
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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