| Author | Silveira, Melise Chaves | |
| Author | Albano, Rodolpho Mattos | |
| Author | Asensi, Marise Dutra | |
| Author | Assef, Ana Paula D'Alincourt Carvalho | |
| Access date | 2016-12-08T14:32:14Z | |
| Available date | 2016-12-08T14:32:14Z | |
| Document date | 2016 | |
| Citation | ASSEF, Ana Paula D'Alincourt Carvalho; et al. Description of genomic islands associated to the multidrug-resistant Pseudomonas aeruginosa clone ST277. Infection, Genetics and Evolution, v.42, p.60-65, 2016. | pt_BR |
| ISSN | 1567-1348 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16484 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Comparações genômicas | pt_BR |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Clone resistente a multidrogas | pt_BR |
| Title | Description of genomic islands associated to the multidrug-resistant Pseudomonas aeruginosa clone ST277 | pt_BR |
| Type | Article | pt_BR |
| Abstract | Multidrug-resistant Pseudomonas aeruginosa clone ST277 is disseminated in Brazil where it is mainly associated
with the presence of metallo-β-lactamase SPM-1. Furthermore, it carries the class I integron In163 and a 16S
rRNA methylase rmtD that confers aminoglycoside resistance. To analyze the genetic characteristics that might
be responsible for the success of this endemic clone, genomes of four P. aeruginosa strains that were isolated in
distinct years and in different Brazilian states were sequenced. The strains differed regarding the presence of
the genes blaSPM-1 and rmtD. Genomic comparisons that included genomes of other clones that have spread
worldwide from this species were also performed. These analyses revealed a 763,863 bp region in the
P. aeruginosa chromosome that concentrates acquired genetic structures comprising two new genomic islands
(PAGI-13 and PAGI-14), a mobile element that could be used for ST277 fingerprinting and a recently reported
Integrative and Conjugative Element (ICE) associated to blaSPM-1. The genetic elements rmtD and In163 are
inserted in PAGI-13 while PAGI-14 has genes encoding proteins related to type III restriction system and phages.
The data reported in this study provide a basis for a clearer understanding of the genetic content of clone ST277
and illustrate the mechanisms that are responsible for the success of these endemic clones. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | Multidrug resistant clone | pt_BR |
| Subject | Genomic comparisons | pt_BR |
| e-ISSN | 1567-7257 | |
| Embargo date | 2030-01-01 | |
