Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16484
Title: Description of genomic islands associated to the multidrug-resistant Pseudomonas aeruginosa clone ST277
Authors: Silveira, Melise Chaves
Albano, Rodolpho Mattos
Asensi, Marise Dutra
Assef, Ana Paula D'Alincourt Carvalho
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Abstract: Multidrug-resistant Pseudomonas aeruginosa clone ST277 is disseminated in Brazil where it is mainly associated with the presence of metallo-β-lactamase SPM-1. Furthermore, it carries the class I integron In163 and a 16S rRNA methylase rmtD that confers aminoglycoside resistance. To analyze the genetic characteristics that might be responsible for the success of this endemic clone, genomes of four P. aeruginosa strains that were isolated in distinct years and in different Brazilian states were sequenced. The strains differed regarding the presence of the genes blaSPM-1 and rmtD. Genomic comparisons that included genomes of other clones that have spread worldwide from this species were also performed. These analyses revealed a 763,863 bp region in the P. aeruginosa chromosome that concentrates acquired genetic structures comprising two new genomic islands (PAGI-13 and PAGI-14), a mobile element that could be used for ST277 fingerprinting and a recently reported Integrative and Conjugative Element (ICE) associated to blaSPM-1. The genetic elements rmtD and In163 are inserted in PAGI-13 while PAGI-14 has genes encoding proteins related to type III restriction system and phages. The data reported in this study provide a basis for a clearer understanding of the genetic content of clone ST277 and illustrate the mechanisms that are responsible for the success of these endemic clones.
Keywords: Pseudomonas aeruginosa
Multidrug resistant clone
Genomic comparisons
keywords: Comparações genômicas
Pseudomonas aeruginosa
Clone resistente a multidrogas
Issue Date: 2016
Publisher: Elsevier
Citation: ASSEF, Ana Paula D'Alincourt Carvalho; et al. Description of genomic islands associated to the multidrug-resistant Pseudomonas aeruginosa clone ST277. Infection, Genetics and Evolution, v.42, p.60-65, 2016.
ISSN: 1567-1348
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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