| Author | Teixeira, R. C. | |
| Author | Baêta, B. A. | |
| Author | Ferreira, J. S. | |
| Author | Medeiros, R. C. | |
| Author | Monteiro, C. M. Maya | |
| Author | Lara, F. A. | |
| Author | Bell-Sakyi, L. | |
| Author | Fonseca, A. H. | |
| Access date | 2016-12-13T12:12:03Z | |
| Available date | 2016-12-13T12:12:03Z | |
| Document date | 2016 | |
| Citation | TEIXEIRA, R. C. et al. Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines. Brazilian Journal of Medical and Biological Research, v.49, n.7, e5211, 9p, 2016. | pt_BR |
| ISSN | 0100-879X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16516 | |
| Language | eng | pt_BR |
| Publisher | Associação Brasileira de Divulgação Científica | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Borrelia burgdorferi | pt_BR |
| Subject in Portuguese | Marcador de membrana fluorescente | pt_BR |
| Subject in Portuguese | Carrapatos | pt_BR |
| Subject in Portuguese | Linhas de células | pt_BR |
| Subject in Portuguese | Fagocitose | pt_BR |
| Title | Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines | pt_BR |
| Type | Article | |
| DOI | 10.1590/1414-431X20165211 | |
| Abstract | This study aimed to describe the association of Borrelia burgdorferi s.s. with ixodid tick cell lines by flow cytometry and fluorescence and confocal microscopy. Spirochetes were stained with a fluorescent membrane marker (PKH67 or PKH26), inoculated into 8 different tick cell lines and incubated at 30°C for 24 h. PKH efficiently stained B. burgdorferi without affecting bacterial viability or motility. Among the tick cell lines tested, the Rhipicephalus appendiculatus cell line RA243 achieved the highest percentage of association/internalization, with both high (90%) and low (10%) concentrations of BSK-H medium in tick cell culture medium. Treatment with cytochalasin D dramatically reduced the average percentage of cells with internalized spirochetes, which passed through a dramatic morphological change during their internalization by the host cell as observed in time-lapse photography. Almost all of the fluorescent bacteria were seen to be inside the tick cells. PKH labeling of borreliae proved to be a reliable and valuable tool to analyze the association of spirochetes with host cells by flow cytometry, confocal and fluorescence microscopy. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | The Tick Cell Biobank. The Pirbright Institute. Pirbright, UK. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil. | pt_BR |
| Subject | Borrelia burgdorferi | pt_BR |
| Subject | Tick cell lines | pt_BR |
| Subject | Phagocytosis | pt_BR |
| Subject | Fluorescent membrane marker | pt_BR |
| e-ISSN | 1414-431X | |
