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PROTEOMIC PROFILE OF BRUCELLA ABORTUS-INFECTED BOVINE CHORIOALLANTOIC MEMBRANE EXPLANTS
Mol, Juliana P. S. et al. | Date Issued: 2016
Author
Mol, Juliana P. S.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Santos, Renato L.
Andrade, Hélida M.
Lage, Andrey P.
Affilliation
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Parasitologia. Belo Horizonte,MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Parasitologia. Belo Horizonte,MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil.
Abstract
Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.
Keywords in Portuguese
Brucelose
Perfil proteômico
Membrana corioalantóica
Bovinos
 
Keywords
Brucella abortus
Proteomic Profile
bovine chorioallantoic membrane (CAM)
 
Publisher
Public Library of Science
Citation
MOL, Juliana P. S. et al. Proteomic Profile of Brucella abortus- Infected Bovine Chorioallantoic Membrane Explants. Plos One, v.11, n.4, e0154209, 16p, Apr. 2016.
DOI
10.1371/journal.pone.0154209
ISSN
1932-6203