| Author | Corrêa, Alex Pauvolid | |
| Author | Morales, Maria Alejandra | |
| Author | Levis, Silvana | |
| Author | Figueiredo, Luis Tadeu Moraes | |
| Author | Lima, Dinair Couto | |
| Author | Campos, Zilca | |
| Author | Nogueira, Marcia Furlan | |
| Author | Silva, Edson Elias da | |
| Author | Nogueira, Rita Maria Ribeiro | |
| Author | Schatzmayr, Hermann Gonçalves | |
| Access date | 2017-01-03T16:24:04Z | |
| Available date | 2017-01-03T16:24:04Z | |
| Document date | 2011 | |
| Citation | CORRÊA, Alex Pauvolid; et al. Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal. Mem Inst Oswaldo Cruz, v.106, n.4, p.467-474, June 2011. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16681 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Jacarés e Crocodilos | pt_BR |
| Subject in Portuguese | Vírus do Nilo Ocidental | pt_BR |
| Subject in Portuguese | Pantanal | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Equinos | pt_BR |
| Subject in Portuguese | Neutralização | pt_BR |
| Title | Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal | pt_BR |
| Type | Article | |
| Abstract | Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of ≥ four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Enfermidades Virales Humanas Dr Julio I Maiztegui. Laboratório de Arbovirus. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Instituto Nacional de Enfermidades Virales Humanas Dr Julio I Maiztegui. Laboratório de Arbovirus. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Ministério da Agricultura Pecuária e Abastecimento. Embrapa Pantanal. Corumbá, MS, Brasil. | pt_BR |
| Affilliation | Ministério da Agricultura Pecuária e Abastecimento. Embrapa Pantanal. Corumbá, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | West Nile virus | pt_BR |
| Subject | Pantanal | pt_BR |
| Subject | neutralisation | pt_BR |
| Subject | equines | pt_BR |
| Subject | caimans | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1678-8060 | |
