Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16681
Title: Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
Authors: Corrêa, Alex Pauvolid
Morales, Maria Alejandra
Levis, Silvana
Figueiredo, Luis Tadeu Moraes
Lima, Dinair Couto
Campos, Zilca
Nogueira, Marcia Furlan
Silva, Edson Elias da
Nogueira, Rita Maria Ribeiro
Schatzmayr, Hermann Gonçalves
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Instituto Nacional de Enfermidades Virales Humanas Dr Julio I Maiztegui. Laboratório de Arbovirus. Buenos Aires, Argentina.
Instituto Nacional de Enfermidades Virales Humanas Dr Julio I Maiztegui. Laboratório de Arbovirus. Buenos Aires, Argentina.
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Ministério da Agricultura Pecuária e Abastecimento. Embrapa Pantanal. Corumbá, MS, Brasil.
Ministério da Agricultura Pecuária e Abastecimento. Embrapa Pantanal. Corumbá, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Abstract: Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of ≥ four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
Keywords: West Nile virus
Pantanal
neutralisation
equines
caimans
Brazil
keywords: Jacarés e Crocodilos
Vírus do Nilo Ocidental
Pantanal
Brasil
Equinos
Neutralização
Issue Date: 2011
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: CORRÊA, Alex Pauvolid; et al. Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal. Mem Inst Oswaldo Cruz, v.106, n.4, p.467-474, June 2011.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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