Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16727
Title: Evaluation of a generic RT-nested-PCR for detection of flaviviruses in suspected fatal cases of dengue infection, Rio de Janeiro, Brazil
Authors: Araújo, Josélio Maria Galvão de
Gomes, Gabriella Mello
Faria, Nieli Rodrigues da Costa
Araújo, Eliane Saraiva Machado de
Filippis, Ana Maria Bispo de
Santos, Flávia Barreto dos
Schatzmayr, Hermann Gonçalves
Nogueira, Rita Maria Ribeiro
Affilliation: Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Laboratório de Biologia Molecular para Doenças Infecciosas e Câncer. Natal. RN, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Abstract: Flaviviruses are significant causes of disease worldwide and can be classified serologically into several antigenic complexes. The purpose of the present study was to evaluate the effectiveness of a generic RT-nested-PCR for detection of flavivirus during a dengue outbreak in Brazil in 2008. A total of 105 serum samples were collected from patients with fatal outcome and examined by generic RT-PCR, conventional RT-PCR, and IgM serology. The generic RT-PCR confirmed 19 of 105 (18%) cases. Conventional RT-PCR performed on 105 serum samples detected 45 (42.8%) dengue virus infections. The IgM serology confirmed 44 of 102 (43.1%) cases. The infecting serotype was identified by generic RT-PCR in 19 cases (18 DENV-2 and 1 DENV-3) and by conventional RT-PCR in 45 cases (40 DENV-2 and 5 DENV-3). In addition, we analyzed the performance of the generic and conventional RT-PCRs and IgM serology on serum samples stratified by the day of onset of symptoms. Our results indicate that different methods should be included in flavivirus surveillance programs, including virological and serological approaches.
Keywords: Dengue
Fatal cases
Brazil
Generic RT-nested-PCR
keywords: Dengue
Casos fatais
Reação em Cadeia da Polimerase
Brasil
Issue Date: 2012
Publisher: Elsevier
Citation: ARAÚJO, Josélio Maria Galvão de; et al. Evaluation of a generic RT-nested-PCR for detection of flaviviruses in suspected fatal cases of dengue infection, Rio de Janeiro, Brazil. Journal of Virological Methods; v.186, n.1-2, p.167-170, 2012.
DOI: 10.1016/j.jviromet.2012.08.010
ISSN: 0166-0934
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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