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Title: Lutzomyia longipalpis saliva triggers lipid body formation and prostaglandin E2 production in murine macrophages
Authors: Santos, Théo de Araújo
Prates, Deboraci Brito
Andrade, Bruno de Bezerril
Nascimento, Danielle de Oliveira
Clarêncio, Jorge
Entringer, Petter F
Carneiro, Alan B
Silva Neto, Mário A. C
Miranda, José Carlos
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Bozza, Patrícia Torres
Borges, Valeria de Matos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro, Brasil
Instituto Nacional de Ciência e Tecnologia. São Paulo, SP, Brasil
Abstract: After the injection of saliva into the host's skin by sand flies, a transient erythematous reaction is observed, which is related to an influx of inflammatory cells and the release of various molecules that actively facilitate the blood meal. It is important to understand the specific mechanisms by which sand fly saliva manipulates the host's inflammatory responses. Herein, we report that saliva from Lutzomyia (L.) longipalpis, a widespread Leishmania vector, induces early production of eicosanoids. Intense formation of intracellular organelles called lipid bodies (LBs) was noted within those cells that migrated to the site of saliva injection. In vitro and ex vivo, sand fly saliva was able to induce LB formation and PGE2 release by macrophages. Interestingly, PGE2 production induced by L. longipalpis saliva was dependent on intracellular mechanisms involving phosphorylation of signaling proteins such as PKC-α and ERK-1/2 and subsequent activation of cyclooxygenase-2. Thus, this study provides new insights into the pharmacological properties of sand fly saliva and opens new opportunities for intervening with the induction of the host's inflammatory pathways by L. longipalpis bites.
keywords: Lutzomya (L.) longipalpis
Leishmaniainfantum chagasi
Resposta inflamatoria
Prostaglandina E2
DeCS: Proteínas e Peptídeos Salivares
Leishmania infantum
Glandulas salivares
Issue Date: 2010
Publisher: Public Library of Science
Citation: ARAÚJO-SANTOS, T. et al.Lutzomyia longipalpis saliva triggers lipid body formation and prostaglandin E2 production in murine macrophages. PLoS Neglected Tropical Diseases, v.4, n.11, p.e873, 2010.
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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