Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17823
Title: A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil
Authors: Portes, Silvana Augusta Rodrigues
Volotão, Eduardo de Mello
Rocha, Monica Simões
Rebelo, Maria Cristina
Xavier, Maria da Penha Trindade Pinheiro
Assis, Rosane Maria de
Rose, Tatiana Lundgren
Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Costa, Filipe Anibal Carvalho
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Laboratório Central de Saúde Pública. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.
Abstract: A that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed.
Keywords: gastroenteritis outbreak
adenovirus
quantitative polymerase chain reaction
Rio de Janeiro
keywords: Gastroenterite
Adenovirus
Reação em Cadeia da Polimerase
surto
Rio de Janeiro
Issue Date: 2016
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: PORTES, Silvana Augusta Rodrigues; et al. A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, v.111, n.6, p.403-406, June 2016.
DOI: 10.1590/0074-02760160030
ISSN: 0074-0276
Copyright: open access
Appears in Collections:PI - Artigos de Periódicos
IOC - Artigos de Periódicos

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