Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17852
Title: Circulating Biomarkers of Immune Activation, Oxidative Stress and Inflammation Characterize Severe Canine Visceral Leishmaniasis
Authors: Solcà, Manuela da Silva
Andrade, Bruno de Bezerril
Abbehusen, Melissa Moura Costa
Teixeira, Clarissa Romero
Khouri, Antonio Ricardo
Valenzuela, Jesus G
Kamhawi, Shaden
Bozza, Patrícia Torres
Fraga, Deborah Bittencourt Mothé
Borges, Valeria Matos
Veras, Patrícia Sampaio Tavares
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Fiocruz Piauí. Teresina, PI, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
National Institute of Allergy and Infectious Diseases. National Institutes of Health. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Twinbrook Parkway, Rockville, USA.
National Institute of Allergy and Infectious Diseases. National Institutes of Health. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Twinbrook Parkway, Rockville, USA.
Instituto Oswaldo Cruz. FIOCRUZ. Bio-Manguinhos. Laboratório de Imunofarmacologia. Rio de Janeiro, Brazil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Departamento de Medicina Veterinária Preventiva e Produção Animal, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia para Doenças Tropicais (INCT-DT). Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia para Doenças Tropicais (INCT-DT). Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (III-INCT). São Paulo, SP, Brasil
Abstract: Clinical manifestations in canine visceral leishmaniasis (CVL) have not been clearly associated with immunological status or disease progression. We simultaneously assessed biomarkers of inflammation, immune activation, oxidative stress, and anti-sand fly saliva IgG concentrations in dog sera with different clinical manifestations to characterize a biosignature associated with CVL severity. In a cross-sectional exploratory study, a random population of 70 dogs from an endemic area in Brazil was classified according to CVL clinical severity and parasitological evaluation. A panel of biomarkers and anti-sand fly saliva IgG were measured in canine sera. Assessment of protein expression of profile biomarkers identified a distinct biosignature that could cluster separately animal groups with different clinical scores. Increasing severity scores were associated with a gradual decrease of LTB4 and PGE2, and a gradual increase in CXCL1 and CCL2. Discriminant analyses revealed that combined assessment of LTB4, PGE2 and CXCL1 was able to distinguish dogs with different clinical scores. Dogs with the highest clinical score values also exhibited high parasite loads and higher concentrations of anti-saliva antibodies. Our findings suggest CVL clinical severity is tightly associated with a distinct inflammatory profile hallmarked by a differential expression of circulating eicosanoids and chemokines.
Keywords: Biomarkers
Canine Visceral Leishmaniasis
Inflammation
Animals
Dogs
Saliva
Fly
keywords: Biomarcadores
Leishmaniose Visceral Canina
Inflamação
Animal
Cães
Saliva
Mosca
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: SOLCÀ, M. S. et al. Circulating Biomarkers of Immune Activation, Oxidative Stress and Inflammation Characterize Severe Canine Visceral Leishmaniasis. Scientific Reports, v. 6, p. 32619, 2016.
DOI: 10.1038/srep32619
ISSN: 2045-2322
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos
PI - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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