Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17894
Title: Differentiation of Trypanosoma cruzi I (TcI) and T. cruzi II (TcII) genotypes using genes encoding serine carboxypeptidases
Authors: Araújo, Catarina Andréa Chaves de
Mayer, Christoph
Waniek, Peter Josef
Azambuja, Patricia
Jansen, Ana Maria
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Museum Alexander Koenig. Bonn, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Abstract: The parasite Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) can be classified based on biochemical and molecular markers, into six lineages or discrete typing units (DTUs), T. cruzi I-VI (TcI-VI), from which TcI and TcII are the parental genotypes. Trying to understand the dispersion of the subpopulations of T. cruzi in nature and its complex transmission cycles, the serine carboxypeptidase genes of T. cruzi were used as a molecular marker in the present study. DTUs of 25 T. cruzi isolates derived from different hosts and from different regions of Brazil were classified. Using specific primers, the complete serine carboxypeptidase open reading frame of 1401 bp was sequenced. The obtained data shows significant differences in the sequences of TcI and TcII. The analysis of the T. cruzi significantly different serine carboxypeptidase genes allowed distinguishing between the parental DTUs TcI to TcII and the hybrid DTU TcVI which grouped within the latter branch. The sequence diversity within the T. cruzi subpopulations was rather low. The analysis using the genes encoding proteases seems to be an interesting approach for the reconstruction of the origin and genotype evolution of T. cruzi.
Keywords: Trypanosoma cruzi I and II
Serine carboxypeptidases
Genotypes
Chagas Disease
Transmission cycles
keywords: Trypanosoma cruzi
Doença de Chagas
Genótipos
carboxipeptidases de serina
Ciclos de transmissão
Issue Date: 2016
Publisher: Springer Verlag
Citation: ARAÚJO, Catarina Andréa Chaves de; et al. Differentiation of Trypanosoma cruzi I (TcI) and T. cruzi II (TcII) genotypes using genes encoding serine carboxypeptidases. Parasitol Res., v.115, n.11, p.4211-4219, 2016.
DOI: 10.1007/s00436-016-5198-8
ISSN: 0932-0113
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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