Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1790
Title: Activity of Physalin F in a Collagen-Induced Arthritis Model
Authors: Brustolim, Daniele
Vasconcelos, Juliana Fraga
Freitas, Luiz Antonio Rodrigues de
Teixeira, Mauro Martins
Farias, Marcel T
Ribeiro, Yvone M
Tomassini, Therezinha Coelho Barbosa
Oliveira, Geraldo Gileno de Sá
Pontes-de-Carvalho, Lain Carlos
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Minas Gerais, BH, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Abstract: The effects of physalin F (1), a steroid derivative purified from Physalis angulata, were investigated in models of collagen-induced arthritis in DBA/1 mice and allergic airway inflammation in BALB/c mice. Oral treatment with 1 or dexamethasone caused a marked decrease in paw edema and joint inflammation when compared to vehicle-treated arthritic mice. In contrast, treatment with 1 had no effect in mice with allergic airway inflammation caused by ovalbumin immunization, whereas dexamethasone significantly reduced the number of inflammatory cells and eosinophils in the broncoalveolar lavage fluid and in lung sections of challenged mice. To further demonstrate that 1 acts through a mechanism different from that of glucocorticoids, a nuclear translocation assay was performed of the glucocorticoid receptor (GR) using COS-7 cells transfected with a plasmid encoding for a yellow fluorescent protein (YFP)-GR fusion protein. Untreated or treated cells with 1 had YFP staining mainly in the cytoplasm, whereas in dexamethasone-treated cells the YFP staining was concentrated in the nuclei. It is concluded that the mechanism of the immunosuppressive activity of physalin F is distinct from that of the glucocorticoids.
DeCS: Antiinflamatórios/PD
Modelos Animas de Doenças
Secoesteróides
Artrite Experimental
Camundongos Endogâmicos BALB C
Issue Date: 2010
Citation: BRUSTOLIM, D. et al. Activity of Physalin F in a Collagen-Induced Arthritis Model. Journal of Natural Products, v.73, n.8, p.1323-1326, 2010.
DOI: 10.1021/np900691w
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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