Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17984
Title: Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil
Authors: Carvalho, Ricardo César Tavares
Vasconcellos, Sidra Ezidio Gonçalves
Issa, Marina de Azevedo
Soares Filho, Paulo Martins
Mota, Pedro Moacyr Pinto Coelho
Araújo, Flábio Ribeiro de
Carvalho, Ana Carolina da Silva
Gomes, Harrison Magdinier
Suffys, Philip Noel
Figueiredo, Eduardo Eustáquio de Souza
Paschoalin, Vânia Margaret Flosi
Affilliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil / Universidade Federal de Mato Grosso. Faculdade de Nutrição. Cuiabá, MT, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ. Brasil.
Ministério da Agricultura, Pecuária e Abastecimento. Laboratório Nacional Agropecuário. Pedro Leopoldo, MG, Brasil.
Ministério da Agricultura, Pecuária e Abastecimento. Laboratório Nacional Agropecuário. Pedro Leopoldo, MG, Brasil.
Ministério da Agricultura, Pecuária e Abastecimento. Laboratório Nacional Agropecuário. Pedro Leopoldo, MG, Brasil.
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte). Campo Grande, MS, Brasil.
Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus Macaé. Macaé, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ. Brasil / Tropical Institute of Medicine. Mycobacteriology Unit. Antwerp, Belgium.
Universidade Federal de Mato Grosso. Faculdade de Nutrição. Cuiabá, MT, Brasil.
Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.
Abstract: Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRU-VNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = ≥ 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.
Keywords: Mycobacterium bovis
Molecular Typing
Midwest Brazil
Cattle Reared
keywords: Tipagem Molecular
Doenças dos Bovinos
Mycobacterium bovis
Centro Oeste
Brasil
Issue Date: 2016
Publisher: Public Library of Science
Citation: CARVALHO, Ricardo César Tavares; et al. Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil. Plos One, v.11, n.9, e0162459, 16p, Sept. 2016.
DOI: 10.1371/journal.pone.0162459
ISSN: 1932-6203
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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